Remarks on mixotrophic and autotrophic carbon nutrition of Vitis plantlets cultured in vitro |
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Authors: | Rose Galzy Daniel Compan |
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Institution: | (1) Chaire de Microbiologie Industrielle et de Génétique des Microorganismes, INRA, Place Viala, 34060 Montpellier Cedex, France |
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Abstract: | Two Vitis species were cultured in vitro under photoautrophic (sucrose-free culture medium) and photomixotrophic (sucrose 15 g l-1) conditions during the period following microcutting rooting (day 34 to day 120). Several parameters were measured at the end of the culture: growth, plant dry weight, carbohydrate uptake from the medium and rates of photosynthesis and dark respiration. The two species behaved very differently. Under photoautotrophic conditions, dark respiration, net photosynthesis and daily CO2 fixation were higher in Vitis vinifera than in Vitis rupestris. Culture under mixotrophic conditions caused increase in growth, respiration and photosynthesis in Vitis rupestris. In contrast, photosynthesis decreased in Vitis vinifera under the same conditions. |
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Keywords: | Vitis grapevine in vitro culture photomixotropy photosynthesis respiration |
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