Direct DNA transfer using electric discharge particle acceleration (ACCELL? technology) |
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Authors: | Dennis McCabe Paul Christou |
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Institution: | (1) Agracetus, Inc., University Green, 8520, 53562 Middleton, WI, USA |
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Abstract: | Direct DNA transfer methods based on particle bombardment have revolutionized plant genetic engineering. Major agronomic crops
previously considered recalcitrant to gene transfer have been engineered using variations of this technology. In many cases
variety-independent and efficient transformation methods have been developed enabling application of molecular biology techniques
to crop improvement. The focus of this article is the development and performance of electric discharge particle bombardment
(ACCELL™) technology. Unique advantages of this methodology compared to alternative propulsion technologies are discussed
in terms of the range of species and genotypes that have been engineered, and the high transformation frequencies for major
agronomic crops that enabled the technology to move from the R&D phase to commercialization.
Creation of transgenic soybeans, cotton, and rice will be used as examples to illustrate the development of variety-independent
and efficient gene transfer methods for most of the major agronomic crops. To our knowledge, no other gene transfer method
based on particle bombardment has resulted in variety-independent and practical generation of large numbers of independently-derived
crop plants. ACCELL™ technology is currently being utilized for the routine transfer of valuable genes into elite germplasm
of soybean, cotton, bean, rice, corn, peanut and woody species. |
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Keywords: | dicot transformation monocot transformation particle bombardment transgenic cotton Gossypium hirsutum transgenic riceOryza sativa transgenic soybeanGlycine max |
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