Genetic manipulation of Japonica rice using the <Emphasis Type="Italic">OsBADH1</Emphasis> gene from Indica rice to improve salinity tolerance |
| |
Authors: | Supaporn Hasthanasombut Kanyaratt Supaibulwatana Masahiro Mii Ikuo Nakamura |
| |
Institution: | (1) Laboratory of Plant Cell Technology, Graduate School of Horticulture, Chiba University, 648 Matsudo, Matsudo City, Chiba 271-8510, Japan;(2) Department of Biotechnology, Faculty of Science, Mahidol University, Rama VI Road, Bangkok, 10400, Thailand; |
| |
Abstract: | Betaine aldehyde dehydrogenase (BADH) is a major oxidative enzyme that converts betaine aldehyde to glycine betaine (GB),
an osmoprotectant compound in plants. Japonica rice (salt-sensitive) was genetically engineered to enhance salt tolerance
by introducing the OsBADH1 gene from Indica rice (salt-tolerant), which is a GB accumulator. We produced transgenic rice plants overexpressing the modified
OsBADH1 gene under the control of the maize ubiquitin promoter. The transgenic rice showed increased OsBADH1 gene expression and OsBADH1 enzyme production, resulting in the accumulation of GB. It also exhibited enhanced salt tolerance
in immature and mature transgenic rice seedlings. The adverse effect of salt stress on seed germination, the growth of immature
and mature seedlings, water status, and photosynthetic pigments was alleviated in transgenic seedlings. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|