Propagation of the tropical tree,Leucaena leucocephala K67, by in vitro bud culture |
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Authors: | Yashpal Goyal R L Bingham Peter Felker |
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Institution: | (1) Caesar Kleberg Wildlife Research Institute, College of Agriculture, Texas A & I University, Campus Box 218, 8363 Kingsville, TX, USA;(2) Present address: 242, Shiv Road, Ratanada, 342001 Jodhpur, Rajasthan, India |
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Abstract: | A tissue culture method is described for clonal multiplication of Leucaena leucocephala K67 using single lateral bud explants from 2–3 m tall greenhouse grown trees. N-6 benzyladenine (BA: 3.0 mg.1-1) and napthaleneacetic acid (NAA: 0.05 mg.1-1) in Murashige & Skoog's (MS) medium were found to be best suited for multiple shoot differentiation in 4–5 week old cultures.
Analysis of variance of the main treatment effects of BA and NAA on shoot parameters showed that BA significantly (P=0.001) affected shoot development while NAA did not. A shoot multiplication rate of 22±3.63 shoots per bud explant was obtained
in 150 days on 1/2 strength MS medium with 3.0 mg.1-1 BA and 0.05 mg.1-1 NAA. Shoots developed adventitious roots within 15 days in 1/2 strength MS medium containing indole-3-butyric acid (IBA:
3.0 mg.1-1) and Kinetin (0.05 mg.1-1). Eighty percent of the transplanted plantlets are being grown in greenhouse conditions. |
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Keywords: | Leucaena leucocephala clonal multiplication single bud culture regeneration in vitro tree legumes |
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