In vitro clonal propagation of tea (Camellia sinensis (L.) O. Kuntze) |
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Authors: | Beena Agarwal Urmi Singh Maitrevi Banerjee |
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Institution: | (1) National Agricultural and Scientific Research Foundation 113/2, Amherst Street, 700 009 Calcutta, India |
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Abstract: | A system for in vitro clonal propagation has been developed in tea plants. Shoots obtained from primary explants were induced from terminal buds and axillary buds of mature field-grown plants. Cultures were initiated from both types of explants on Murashige and Skoog (MS) medium supplemented with 10% coconut milk (CM), 200 mg l-1 of yeast extract (YE), 1.4 M indoleacetic acid (IAA) and 17.8 M benzyladenine (BA). The shoot tips were multiplied on 1/2 strength MS medium containing 10% CM, 2.9 M IAA and 17.8 M BA. The larger shoots were separated after multiplication and rooted on 1/2 MS medium supplemented with 11.4 M ascorbic acid and 34.5 M indolebutyric acid (IBA). A pretreatment of the plants with an aqueous solution of 493 M IBA greatly increased the frequency of rooting. More than 60% of the rooted plants have been transferred to soil successfully.Abbreviations BA
benzyladenine
- IAA
indoleacetic acid
- IBA
indolebutyric acid
- YE
yeast extract
- CM
coconut milk
- MS
Murashige and Skoog medium (1962) |
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Keywords: | micropropagation terminal and axillary bud whole plant |
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