Production of microspore-derived plants by anther culture of an interspecific F1 hybrid between Cyclamen persicum and C. purpurascens |
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Authors: | Ishizaka Hiroshi |
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Institution: | (1) Saitama Horticultural Experiment Station, 91 Rokumanbu, Kuki, Saitama, 346-0037, Japan |
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Abstract: | Anthers of a F1 hybrid (2n=41) between Cyclamen persicum (2n=2x=48) and C. purpurascens (2n=2x=34) were cultured to produce
microspore-derived plants. Embryoids were produced when anthers, containing microspores at the early uninucleate stage of
pollen development, were cultured in B5 medium containing sucrose (90 g l-1) and NAA (0.1, 1 mg l-1) or 2,4-D (0.1 mg l-1)
in the dark at 5 °C for 4 days, then at 25 °C for 60 days. The embryoids usually developed into plantlets when cultured in
B5 medium containing sucrose (30 g l-1) in the dark at 25 °C. At meiosis, the F1 hybrid, used as source for anther culture,
formed some cells with restitution nuclei at telophase and dyads at the tetrad stage, which resulted in the production of
viable pollen grains as unreduced gametes. Plants produced by anther culture were grouped into sterile plants with 2n=41 chromosomes
and fertile plants with 2n=82 chromosomes. The present findings suggested that the sterile plants were polyhaploids originating
from unreduced microspores (n=41) of the F1 hybrid and that the fertile plants were amphidiploids induced by a spontaneous
doubling during culture of chromosomes of such unreduced microspores.
This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | amphidiploid anther culture Cyclamen persicum - C purpurascens F1 hybrid polyhaploid unreduced microspore |
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