Barley spikelet culture: An effective tool for the analysis of biochemical events in flower development |
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| Authors: | James D Astwood Robert D Hill |
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| Institution: | (1) Department of Plant Science, University of Manitoba, R3T 2N2 Winnipeg, Manitoba, Canada;(2) Present address: Department of Horticulture and Landscape Architecture, Washington State University, 99164-6414 Pullman, Washington |
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| Abstract: | Immature detached barley spikelets were cultured in wheat spikelet medium. Fertility of cultured barley spikelets was similar to that of cultured wheat spikelets. Barley anther development within cultured spikelets was retarded relative to in planta, but viability of developing pollen, as determined by a fluorochromatic reaction, was similar in vitro and in planta. Protein synthesis by anthers in developing barley spikelets in vitro was maximal during stage 2, when pollen grains were bicellular, and declined as pollen matured. Barley spikelet culture is an effective tool for the analysis of biochemical events in flower development.Abbreviations FRC
fluorochromatic reaction
- WSM
wheat spikelet medium |
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| Keywords: | anther barley pollen protein spikelet culture |
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