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Rapid propagation of tuna (Opuntia ficus-indica) and plant establishment in soil |
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| Authors: | Yasseen Mohamed-Yasseen Sheryl A Barringer Walter E Splittstoesser Raymond J Schnell |
| Institution: | (1) Dept. of Horticulture, University of Illinois, S-406 Turner Hall, 1102 S. Goodwin Ave., 61801 Urbana, IL, USA;(2) Dept. of Food Sci & Tech., Ohio State University, 122 Vivian Hall, 2121 Fyffe Rd., 43210-1097 Columbus, OH, USA;(3) Nat. Clonal Germplasm Rep., USDA, ARS, 13601 Old Cutler Rd., 33158 Miami, FL |
| Abstract: | Explants from young joints of mature plants of tuna (Opuntia ficus-indica Mill.) were cultured on Murashige and Skoog (MS) medium containing 8.8  M benzyladenine (BA) and 0.5 M naphthaleneacetic acid (NAA). Shoots produced were utilized as secondary explants. Each shoot was cut longitudinally from apex to base into two explants, and some of these explants were cut transversely into proximal and distal explants. The size and number of shoots produced was affected by size and position of the explant within its source. The shoots were rooted in vitro or ex vitro and plants were successfully established in soil from both rooting methods.Abbreviations AC
activated charcoal
- BA
benzyladenine
- IBA
indolebutyric acid
- MS
Murashige & Skoog (1962) medium
- NAA
naphthaleneacetic acid |
| Keywords: | Cactus prickly pear micropropagation Indian fig organogenesis |
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