Tissue culture independent transformation for Corchorus olitorius |
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Authors: | Abu Ashfaqur Sajib Md Shahidul Islam Md Shamim Reza Arpita Bhowmik Layla Fatema Haseena Khan |
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Institution: | (1) School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, 230009, The People’s Republic of China |
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Abstract: | An efficient microprogation protocol has been developed for Dendrobium
densiflorum Lindl. ex Wall., a traditional medicinal plant, through protocorm-like bodies (PLBs) from nodal stem segments using 6-benzylamino-purine
(BAP) and the lanthanoid neodymium. The highest percentage of explants producing PLBs (72%), with an average of 15 PLBs per
explant, was induced by culturing stem segments on Murashige and Skoog (MS) medium supplemented with 5.0 mg l−1 BAP. The newly formed PLBs proliferated well on the basal MS medium and completely converted into shoots on MS medium containing
2.0 mg l−1 BAP. Shoots produced an average of 22 roots per plantlet when cultured on MS medium supplemented with 2.0 mg l−1 neodymium nitrate. Healthy plantlets with well-developed roots were successfully acclimatized. The obtained result suggests
that the lanthanoids can be used to effectively initiate rooting in the micropropagation and conservation of D. densiflorum. |
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Keywords: | Dendrobium densiflorum Medicinal plant Micropropagation Protocorm-like bodies Lanthanoids Rare earth elements |
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