Effect of high salinity on tracheary element differentiation in light-grown callus of Mesembryanthemum crystallinum |
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Authors: | Yen Hungchen Emilie Yen Shi-Kae |
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Institution: | (1) Department of Botany, National Chung-Hsing University, Taichung, Taiwan, 40227, Republic of China ( |
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Abstract: | This study investigated the inhibitory effects of NaCl on tracheary element (TE) differentiation in light-grown callus of
ice plant Mesembryanthemum crystallinum L., a halophyte which adaptes well to saline environments. When ice plant callus was grown in a modified Linsmaier-Bednar
and Skoog culture medium containing no NaCl (control medium), up to 20% of ice plant cells differentiated into tracheary elements
during in vitro culture. Close examination of callus tissues stained with potassium permanganate revealed that tracheary elements were aggregated
as discrete nodules. Some strikingly elongated tracheary elements were found in the macerated tissues. Experimental results
indicated that adding 200 mM NaCl to the control medium reversibly inhibited the formation of tracheary element in the halophytic
cells. The rate of tracheary element formation increased accordingly as the rate of cell growth in control medium. In the
presence of high salt, the degree of tracheary element differentation remained low through the growth cycle. The inhibitory
effect of salt on tracheary element differentiation was overcome by adding 10 mg l−1 salicylic acid, a known signaling compound that induces a diverse group of defense-related genes, including genes involved
in reinforcing the host cell wall. Furthermore, microscopic examination revealed that most tracheary elements formed under
this treatment (200 mM NaCl plus 10 mg l−1 salicylic acid) were round shaped. The results suggest that high salt inhibits both the biosynthesis of secondary wall components
and cell elongation ice plant in vitro culture.
This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | cytodifferentiation ice plant salicylic acid salt stress tracheary element |
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