Indirect shoot organogenesis from leaves of Dieffenbachia cv. Camouflage |
| |
Authors: | Xiuli Shen Jianjun Chen Michael E Kane |
| |
Institution: | (1) Department of Environmental Horticulture, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611-067, USA;(2) Mid-Florida Research and Education Center, Institute of Food and Agricultural Sciences, University of Florida, 2725 Binion Road, Apopka, FL 32703-8504, USA |
| |
Abstract: | A novel protocol for indirect shoot organogenesis of Dieffenbachia cv. Camouflage was established using leaf explants excised from in vitro shoot cultures. The frequency of callus formation
reached 96% for explants cultured on Murashige and Skoog (1962) basal medium supplemented with 5 μM thidiazuron and 1 μM 2,4-dichlorophenozyacetic acid. The number of shoots regenerated
was high, with up to 7.9 shoots produced per callus cultured on basal medium supplemented with 40 μM N
6-(Δ2-isopentenyl)adenine and 2 μM indole-3-acetic acid. Regenerated shoots rooted well in a soilless substrate, acclimatized ex
vitro at 100%, and grew vigorously under shaded greenhouse conditions. Somaclonal variations in leaf variegation, color, and
morphology have been observed in regenerated plants. |
| |
Keywords: | Dieffenbachia Ornamental foliage plants Shoot organogenesis |
本文献已被 SpringerLink 等数据库收录! |
|