Anther culture response of Hordeum spontaneum-derived winter barley lines

The anther culture response of 18 Hordeum spontaneum derived winter barley lines, as well as that of the F₂ and/or BC₁ progeny of the cross between nine of these lines and the winter barley cultivar Triton was assessed. While the majority of lines showed a poor regeneration capacity (4%), two lines, RS 170-A13 × Sonja and RS 120-27 × Sonja demonstrated an outstanding response, producing 26 and 8 green regenerants per 100 anthers plated, respectively. Five further lines, RS 70-30 × Dura, RS L74-11 × Dura, RS 122-19 × Sonja. RS 142-29 × Dura and RS 170-A51 × Sonja were identified as highly responsive in the F₂ generation. An average production of 4 green regenerants per 100 anthers plated was observed both in the parental and F₂ generation.Abbreviations BA benzyladenine - IAA indole-3-acetic acid