Peroxidase activity in calluses and cell suspension cultures of radishRaphanus sativus var. Cherry Bell |
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| Authors: | Oscar A Moreno V Rafael Vazquez-Duhalt |
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| Institution: | (1) Centro de Investigaciones Biológicas de Baja California Sur, Apartado Postal 128, 23000 La Paz, B.C.S., México |
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| Abstract: | Calluses ofRaphanus sativus var. Cherry Bell were induced in a medium containing 2,4-dichlorophenoxyacetic acid and benzyladenine. The biomass and peroxidase activities were determined, both in agar cultures and cell suspension cultures. Different growth-regulator concentrations induced different responses measured as peroxidase activity in callus. The suspended-cell cultures showed the importance of selecting the cell line, in order to obtain an optimal response in extracellular peroxidase activity. The commercial production of this enzyme by utilizing plant cell tissue cultures is discussed. |
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| Keywords: | Raphanus sativus peroxidase callus cell suspension extracellular enzyme |
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