Evaluation of the genetic fidelity of in vitro-propagated gerbera (<Emphasis Type="Italic">Gerbera jamesonii</Emphasis> Bolus) using DNA-based markers |
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Authors: | R Bhatia K P Singh T R Sharma Tripta Jhang |
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Institution: | (1) Indian Agricultural Research Institute, Regional Station, Katrain, Kulluvalley, Himachal Pradesh, 175129, India;(2) Division of Floriculture and Landscaping, Indian Agricultural Research Institute, New Delhi, 110012, India;(3) National Research Center on Plant Biotechnology, Indian Agricultural Research Institute, New Delhi, 110012, India |
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Abstract: | The genetic fidelity of in vitro-raised gerbera clones was assessed by using random amplified polymorphic DNA (RAPD) and inter-simple
sequence repeat (ISSR) markers. Out of 35 RAPD and 32 ISSR primers screened, only 12 RAPD and 10 ISSR primers produced clear,
reproducible and scorable bands. The 12 RAPD primers produced 54 distinct and scorable bands, with an average of 4.5 bands
per primer. The number of scorable bands for ISSR primers varied from 3 (ISSR-14) to 9 (ISSR-07), with an average of 5.5 bands
per primer. The number of bands generated per primer was greater in ISSR than RAPD. All banding profiles from micropropagated
plants were monomorphic and similar to those of the mother plant. A similarity matrix based on Jaccard’s coefficient revealed
that the pair-wise value between the mother and the in vitro-raised plantlets was 1, indicating 100% similarity. This confirmed
the true-to-type nature of the in vitro-raised clones. |
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