Callus induction and plantlet regeneration in damiana (Turnera diffusa,Willd.) |
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Authors: | Antonio J Díaz-Rondero Lilia Alcaraz-Meléndez |
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Institution: | (1) Div. de Biología Experimental, Centro de Investigaciones Biológicas de Baja California Sur, Baja California Sur, Apdo. Postal 128, 23000 La Paz, México |
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Abstract: | Experiments were performed to determine optimal conditions for callus induction and plantlet regeneration from damiana (Turnera diffusa, Willd.) leaf explants. MS media 7] and B5 media 4] were used; both of them were supplemented with different combinations of 2,4-dichlorophenoxyacetic, acid (2,4-D) and 6-benzyl-adenine (BA). In all cases the samples were incubated under continous fluorescent light, 100 mol.m-2.sec-1, at 25±2°C for 60 days. Highest callus percentage was obtained in MS media supplemented with 2,4-D 10–5 M plus BA 10-8M. Maximum percentage and multiple shoots and leaves were produced in MS media with BA 10-5M and BA 10-5M plus 2,4-D 10-7M. These explants were transferred for rooting to MS medium without plant growth regulators. Later, rooted plantlets were successfully established in soil. |
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Keywords: | Turnera diffusa damiana vegetative propagation callus culture |
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