Community analysis of arbuscular mycorrhizal fungi in a warm-temperate deciduous broad-leaved forest and introduction of the fungal community into the seedlings of indigenous woody plants |
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Authors: | Masahide Yamato Koji Iwase |
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Institution: | (1) Biological Environment Institute, The General Environmental Technos Co., Ltd., 8-4 Ujimatafuri, Uji, Kyoto 611-0021, Japan |
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Abstract: | A community of arbuscular mycorrhizal (AM) fungi was investigated in a warm-temperate deciduous broad-leaved forest using
a molecular analysis method. Root samples were obtained from the forest, and DNA was extracted from the samples. Partial 18S
rDNA of AM fungi were amplified from the extracted DNA by polymerase chain reaction using a universal eukaryotic primer NS31
and an AM fungal-specific primer AM1. After cloning the PCR products, 394 clones were obtained in total, which were divided
into five types by restriction fragment length polymorphism (RFLP) with HinfI, RsaI, and Hsp92II. More than 20% of the clones were randomly selected from each RFLP type and sequenced. Phylogenetic analysis showed that
all the obtained clones belonged to Glomus but could not be identified at species level. Topsoil of the forest containing plant roots was inoculated to nonmycorrhizal
seedlings of indigenous woody plants, Rhus javanica var. roxburghii and Clethra barvinervis, to introduce the community of AM fungi into the seedlings. Among these five RFLP types, four types were detected from both
seedlings, which indicates that the AM fungal community in the forest root samples was introduced at least partly into the
seedlings. Meanwhile, an additional four types that were not found in the forest root samples were newly detected in the seedlings,
these types were closely related to one another and close to G. fasciculatum or G. intraradices. It is expected that a community of indigenous diverse AM fungi could be introduced into target fields by planting these
mycorrhizal seedlings. |
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Keywords: | Arbuscular mycorrhiza Clethra barvinervis Community structure Deciduous broad-leaved forest Rhus javanica var roxburghii |
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