Dynamics of the actin cytoskeleton,hyphal tip growth and the movement of the two nuclei in the dikaryon ofCoprinus cinereus |
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Authors: | Shigeru Tanabe Takashi Kamada |
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Institution: | (1) Department of Biology, Faculty of Science, Okayama University, 700 Okayama, Japan;(2) Present address: Institute of Genetic Ecology, Tohoku University, 2-1-1, Katahira, Aoba-ku, 980-77 Sendai, Japan |
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Abstract: | We first examined the changes in distribution of F-actin during conjugate division in the apical cells of the dikaryon ofCoprinus cinereus using indirect immunofluorescence microscopy, then followed hyphal tip growth and the movement of the two nuclei in the apical
cells using differential interference contrast microscopy (DIC). In apical cells with interphase nuclei, F-actin occurred
solely as peripheral plaques, which were distributed along the whole length of the cell and were more concentrated at the
tips, where they formed caps. In the early prophase of conjugate division, F-actin was transiently concentrated, as diffused
form and plaques, at hyphal regions where the two nuclei sit, and this was accompanied by transient disappearance of the actin
cap at the hyphal tip in the majority of cells. The actin cap was also present at the tips of growing clamp cells from late
prophase through metaphase and disintegrated during anaphase. In telophase, actin rings formed at the future septa. DIC revealed
that, in early prophase, when the F-actin array occurs around the two nuclei and the actin cap is absent at hyphal tips, hyphae
kept growing and the second nucleus accelerated its forward movement to catch up with the leading nucleus, which was still
moving forward. |
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Keywords: | actin conjugate division Coprinus cinereus nuclear movement tip growth |
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