Characterization of the carbohydrase productions of an ectomycorrhizal fungus, Tricholoma matsutake |
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Authors: | Mizuho Kusuda Mitsuhiro Ueda Kazutaka Miyatake Takao Terashita |
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Institution: | (1) Laboratory of Biocycle Engineering, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka, Japan;(2) Laboratory of Food Microbiological Science and Biotechnology, School of Agriculture, Kin-ki University, 3327-204, Nakamachi, Nara 631-8505, Japan |
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Abstract: | To evaluate the potential of the production of the ectomycorrhizal fungus Tricholoma matsutake to produce carbohydrases, (1) the distribution of carbohydrase activities among the different strains (18 strains) was investigated
and (2) the abilities of T. matsutake and saprophytic fungi to produce β-glucosidase were compared. The results showed that the carbohydrase productions patterns
of T. matsutake still resemble one another. Moreover, this fungus exhibited markedly higher β-glucosidase than did the saprophytic mushrooms.
Tricholoma matsutake showed weak production of α-amylase and α-glucosidase in a static cultur filtrate. On the other hand, glucoamylase activity
was not observed. Surprisingly, we discovered that β-glucosidase demonstrated strong activity. This finding suggests that
this fungus has saprotrophic abilities. The carbohydrase production systems in T. matsutake were characterized from our experimental results. Also, we point out some weak points in the carbohydrase production systems
of T. matsutake. |
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Keywords: | β -Glucosidase Carbohydrase Ectomycorrhizal fungus Edible mushroom Tricholoma matsutake |
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