印楝愈伤组织形成及其印楝素含量测定

取印楝（AzadirachtaindicaA．Juss）不同器官作外植体，研究培养基和继代次数对愈伤组织生长及其印楝素（Azadirachtin）生物合成的影响．印楝的不同器官（根、叶、茎及皮）均能诱导出愈伤组织，这些愈伤组织均有合成印楝素的能力．其中以叶诱导的愈伤组织生长速率及印楝素含量为最高．含有较低按盐的B5培养基有利于细胞生长，含有较高铵盐的MS培养基有利于印楝素积累，不含铰盐的White培养基对两者均不利．愈伤组织继代2—3代，有利于愈伤组织生长和印楝素合成。

CALLUS FORMATION IN EXPLANTS OF AZADIRACHTA INDICA AND THE AZADIRACHTIN CONTENT

The influences of different explants from organs of Azadirachta indica, culture media and subculture times on callus growth and azadirachtin content in cultures were studied. The results showed that all of the calli induced from root, leaf, stem and bark had the capability of synthesizing azadirachtin, among which callus growth rate and azadirachtin from leaf explants were the highest. BS medium with low anunonium salt had good result for callus growth, while MS medium with high ammonium salt was beneficial for azadirachtin accumulation, but White medium without anunonium salt was satisfactory for neither of them. The best callus growth and azadirachtin formation occurred at the second or third subculture.