| 醮核荔枝胚胎发育相关蛋白质的分离及初步鉴定 |
| |
| 引用本文： | 李蕾,彭存智,李明芳,王向社,郑学勤.醮核荔枝胚胎发育相关蛋白质的分离及初步鉴定[J].热带亚热带植物学报,2008,16(6):537-544. |
| |
| 作者姓名： | 李蕾 彭存智 李明芳 王向社 郑学勤 |
| |
| 作者单位： | 1. 海南师范大学生物系,海口,571158;中国热带农业科学院热带生物技术研究所,海口,571101
2. 中国热带农业科学院热带生物技术研究所,海口,571101 |
| |
| 基金项目： | 863计划项目(2004AA001380); 海南省自然科学基金项目(80419); 海南省教育厅高校科学研究项目(HJKJ200740); 海南师范大学植物学重点扶持学科项目资助 |
| |
| 摘 要： | 通过二维聚丙烯酰胺凝胶电泳以及计算机辅助的图像分析技术，对荔枝开花后40d的正常与败育胚蛋白质图谱进行初步分析。结果表明，100个蛋白质点在表达丰度上有明显差异；选择仅在正常发育胚胎胶上表达的蛋白点15个和仅在败育胚胎胶上表达的蛋白点50个，进行基质辅助激光解吸附电离飞行时间质谱（MALDI—TOFMASS）分析，鉴定出9个与胚发育相关的蛋白，这些蛋白可能参与了胚败育的调节和控制。
|
| 关 键 词： | 荔枝 胚发育 蛋白质图谱 质谱 |
| 收稿时间： | 2007/12/25 0:00:00 |
| 修稿时间： | 2008/7/10 0:00:00 |
Isolation and Identification of Proteins Related with Embryo Development in Seedless Litchi |
| |
| li lei,PENG Cun-zhi,LI Ming-fang,WANG Xiang-she and ZHENG Xue-qing.Isolation and Identification of Proteins Related with Embryo Development in Seedless Litchi[J].Journal of Tropical and Subtropical Botany,2008,16(6):537-544. |
| |
| Authors: | li lei PENG Cun-zhi LI Ming-fang WANG Xiang-she and ZHENG Xue-qing |
| |
| Institution: | Department of Biology, Hainan Normal University,Institute of Tropical Biotechnology and Bioscience, Chinese Academy of Tropical Agriculture Sciences,Institute of Tropical Biotechnology and Bioscience, Chinese Academy of Tropical Agriculture Sciences,Institute of Tropical Biotechnology and Bioscience, Chinese Academy of Tropical Agriculture Sciences,Institute of Tropical Biotechnology and Bioscience, Chinese Academy of Tropical Agriculture Sciences |
| |
| Abstract: | The global protein patterns in normal and aborted embryo of litchi at 40 days after flowering(DAA) were compared by using two-dimensional polyacrylamide gel electrophoresis(2-DE) and computer image analysis.The results showed that the quantities of 100 protein spots in normal embryo were significantly different from aborted embryo.There were 15 and 50 spots appeared on the gel of normal embryo and aborted embryo,respectively.Then they were selected for MALDI-TOF-MASS analysis.Nine proteins related with embr... |
| |
| Keywords: | Litchi Embryo development Proteomics mapping MALDI-TOF-MASS |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《热带亚热带植物学报》浏览原始摘要信息 |
| 点击此处可从《热带亚热带植物学报》下载免费的PDF全文 |
