琴叶风吹楠资源遗传多样性的AFLP分析

为研究濒危植物琴叶风吹楠(Horsfieldia pandurifolia)的遗传多样性,利用AFLP分子标记技术,对采自云南省西双版纳州、临沧市的8个居群共56份琴叶风吹楠样品进行了分析。结果表明,琴叶风吹楠在物种水平的多态性较高,多态性百分率为75.16%;在居群水平,平均多态性百分率为36.20%;AMOVA分析表明,琴叶风吹楠的遗传变异主要存在于居群内(75.45%),而居群间的变异仅为24.55%;mantel检验结果表明,地理距离和遗传距离存在不显著的正相关(r=0.119 7, P=0.321 0);基于遗传相似性系数,对8个居群进行了UPGMA聚类分析,在遗传相似性系数0.951处可将8个居群聚为3组。这些为琴叶风吹楠的保护和开发利用提供了理论依据,并提出了保护建议。

Genetic Diversity of Horsfieldia pandurifolia Based on AFLP Markers

In order to understand the genetic diversity of endangered Horsfieldia pandurifolia, the genetic structure of 56 samples from 8 populations from Xishuangbanna and Lincang of Yunnan Province were studied by using AFLP markers. The results showed that the polymorphisms of H. pandurifolia were high with the percentage of polymorphic sites of 75.16% at species level, and 36.20% at population level. Moreover, the genetic variation (75.45%) within the population was high through AMOVA analysis, but only 24.55% among populations. Based on mantel test, there was not significant positive correlation (r=0.1197, P=0.3210) between geographical distance and genetic distance. According to genetic similarity coefficient at 0.951, the 8 populations could be divided into 3 groups. So, these would provide a foundation for the development and sustainable development of H. pandurifolia, and the conservation strategy of H. pandurifolia germplasms was proposed.