灵芝浸出液在香蕉组织培养中的抑菌作用

设计不同浓度的灵芝浸出液培养基,以香蕉(Musa sapientumL.)作为培养材料,通过定量接种不同种类的细菌及真菌以观察灵芝浸出液的抑菌效果。结果表明,采用野生灵芝10 g于1 000 ml蒸馏水中煮2 h的浸出液配制的培养基,不仅能使香蕉正常生长,而且已感染细菌的材料经一段时间培养后,细菌污染消失,恢复正常。研究结果对于香蕉转基因操作以及完善组织培养技术具有重要的参考意义。

Inhibitory Effects of Ganoderma lucidum Extraction Tissue Culture of Common Banana

The experiments of the effects of Ganoderma lucidum(Leyss.ex Fr.) Karst.extract(GLE) on bacterial inhibition in tissue culture of common banana was carried out by adding various concentrations of GLE into MS medium containing 2 mg L-1 BA and 0.1 mg L-1 NAA.Explants from absorptive buds of common banana(Musa sapientum L.) were used to observe the inhibition of bacteria(Escherichia coli I,Saccharomyces cerevisiae,Staphylococcus aureus,Streptomyces griseus and Agrobacterium tumefaciens),the shooting and the growth of plantlets.It was indicated that best bacterial inhibition concentrations of GLE in primary culture and subcultures were 5 or 10 g Ganoderma pieces boiled in 1 000 ml distilled water for two hours.However,GLE showed certain inhibitory effect on shooting,but there was no influence on rooting.