花生种子老化相关蛋白质的初步研究

本文以粤油 116花生(Arachis hypogaea L.)为材料,对不同处理种子的除子叶“种胚”(以下简称“种胚”)的蛋白质进行了研究.实验结果表明,当花生种子活力下降到一定程度时,其“种胚”内出现一种新蛋白质( pI6.2、MW 10 KD),随种子老化程度加深,含量逐渐增多.我们认为该蛋白质与花生种子老化存在着一定的相关关系,可作为该种子老化的标志.

STUDIES OF PROTEINS RELATED TO PEANUT (ARACHIS HYPOGAEA) SEED AGING

Seed aging involves many changes, such as, enzyme activities, integrities of membrances and nuclear acids and others, which result in the loss of seed viability finally, but these can not explain the mechanism. This paper deals with changes of proteins in cotyledonless embryos of peanut (Arachis hypogaed) seeds with various treatments by means of two dimensional gel electrophoresis. The results indicated that a new protein (pI 6.2, MW 10 KD) appeared in the dry aged cotyledonless embryos, which did not exist in the highly viable embryos, regardless of naturally or artificially aged peanut seeds. Morever, the content of the protein increased with intensity of the seed aging. The protein was considered as specific one to the peanut seed aging.