| 蓝花楹组织培养与快速繁殖研究 |
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| 引用本文： | 阳莉,石大兴,麦苗苗,王米力.蓝花楹组织培养与快速繁殖研究[J].热带亚热带植物学报,2012,20(1):26-32. |
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| 作者姓名： | 阳莉 石大兴 麦苗苗 王米力 |
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| 作者单位： | 1. 内江师范学院张大千美术学院,四川 内江,641100
2. 四川农业大学林学院,四川 雅安,625014 |
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| 基金项目： | 森林培育学四川省重点学科建设项目(SZD0419)资助 |
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| 摘 要： | 以蓝花楹(Jacaranda mimosifolia Humb.et Bonpl.)胚轴为外植体进行组织培养和快繁体系建立的研究。结果表明,蓝花楹种子经40℃-45℃温水浸泡后发芽率较高,达到55.7%。蓝花楹不定芽和愈伤组织诱导的最适培养基分别为MS+6-BA2.0 mg L-1+NAA 0.1 mg L-1+2,4-D 0.1 mg L-1和M S+6-BA 0.5 mg L-1+NAA 1.0 mg L-1+2,4-D 1.0 mg L-1。不定芽和愈伤组织增殖的最适培养基分别为改良MS培养基+6-BA 0.5 mg L-1+NAA 0.5 mg L-1+IBA 0.5 mg L-1和MS+6-BA 1.0 mg L-1+NAA 0.5 mg L-1+ZT 3.0 mg L-1。愈伤组织分化最适培养基为M S+BA 1.0 mg L-1+NAA 0.5 mg L-1+2,4-D 0.5 mg L-1。最适生根培养基为1/2MS+蔗糖20 g L-1+NAA 0.1 mg L-1+活性炭2.0 g L-1,生根率达78.3%。
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| 关 键 词： | 蓝花楹 下胚轴 愈伤组织 组织培养 快速繁殖 |
| 收稿时间： | 2011/5/19 0:00:00 |
| 修稿时间： | 2011/7/28 0:00:00 |
Tissue Culture and Rapid Propagation of Jacaranda mimosifolia |
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| YANG Li,SHI Da-xing,MAI Miao-miao and WANG Mi-li.Tissue Culture and Rapid Propagation of Jacaranda mimosifolia[J].Journal of Tropical and Subtropical Botany,2012,20(1):26-32. |
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| Authors: | YANG Li SHI Da-xing MAI Miao-miao and WANG Mi-li |
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| Institution: | Chang Academy of Fine Arts, Neijiang Normal University, China;College of Forestry, Sichuan Agricultural University, China;College of Forestry, Sichuan Agricultural University, China;College of Forestry, Sichuan Agricultural University, China |
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| Abstract: | The hypocotyls of Jacaranda mimosaefolia used as explants,the tissue culture and rapid propagation system were studied.The results showed that the seed germination rate of J.mimosaefolia reached up to 55.6% after warm water immersion for 60 minutes at 40℃-45℃.The optimum mediums for induction of adventitious buds and callus were MS+6-BA 2.0 mg L-1+NAA 0.1 mg L-1+2,4-D 0.1 mg L-1,and MS+6-BA 0.5 mg L-1+NAA 1.0 mg L-1+2,4-D 1.0 mg L-1,respectively.The optimum mediums for proliferation of adventitious buds and callus were modified MS+6-BA 0.5 mg L-1+NAA 0.5 mg L-1+IBA 0.5 mg L-1,and MS+6-BA 1.0 mg L-1+NAA 0.5 mg L-1+ZT 3.0 mg L-1,respectively.The optimum medium for callus differentiation was MS+6-BA 1.0 mg L-1+NAA 0.5 mg L-1+2,4-D 0.5 mg L-1.The optimum rooting medium was 1/2MS supplemented with 20 g L-1 sugar and NAA 0.5 mg L-1 and 2.0 g L-1 active carbon(AC) with rooting up to 78.3%. |
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| Keywords: | Jacaranda mimosifolia Hypocotyls Callus Tissue culture Rapid propagation |
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