| 被子植物未受精胚珠与子房离体培养的研究进展 |
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| 引用本文： | 伍成厚,梁承邺,叶秀麟.被子植物未受精胚珠与子房离体培养的研究进展[J].热带亚热带植物学报,2004,12(6):580-586. |
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| 作者姓名： | 伍成厚 梁承邺 叶秀麟 |
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| 作者单位： | 1. 中国科学院华南植物园,广东,广州,510650;漳州师范学院生物系,福建,漳州,363000
2. 中国科学院华南植物园,广东,广州,510650 |
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| 基金项目： | 广东省科技计划项目(C20304和2002A2040801)资助 |
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| 摘 要： | 介绍被子植物未受精胚珠与子房离体培养诱导单倍体植株的研究进展。迄今已有9个科21种植物用这一方法诱导出单倍体植株，植物的基因型、外植体的发育程度、接种前的预处理、培养基和培养条件等均能影响诱导率的高低。胚胎学观察揭示大孢子与胚囊内的卵细胞、助细胞和反足细胞均有可能在培养中启动分裂，通过胚状体或愈伤组织形成单倍体植株。本项技术在植物单倍体育种中可发挥重要作用。
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| 关 键 词： | 胚珠 子房 愈伤组织 胚状体 单倍体 综述 |
| 文章编号： | 1005-3395(2004)06-0580-07 |
| 修稿时间： | 2003年9月30日 |
Progress in Studies on in vitro Culture of Unfertilized Ovules and Ovaries in Angiosperms |
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| WU Cheng-hou,LIANG Cheng-ye,YE Xiu-lin.Progress in Studies on in vitro Culture of Unfertilized Ovules and Ovaries in Angiosperms[J].Journal of Tropical and Subtropical Botany,2004,12(6):580-586. |
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| Authors: | WU Cheng-hou LIANG Cheng-ye YE Xiu-lin |
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| Abstract: | Gynogenic haploids through unfertilized ovules and ovaries have been reported from 21 species in 9 families since 1976. This review includes the following subjects: induction frequency of haploids depend on genotypes, developmental stage of explants, temperature pretreatment of the explants, medium composition and the conditions of cultivation. Embryological studies reveal that megaspore and such cells as egg, synergid and antipodals in mature embryo sac initiate the division of forming haploid plants through embryogenesis or callus formation. This technique provides an optimal approach to haploid breeding. |
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| Keywords: | Ovule Ovary Callus Embryoid Haploid Review |
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