| 流式细胞术在高等植物研究中的应用 |
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| 引用本文： | 焦旭雯,赵树进.流式细胞术在高等植物研究中的应用[J].热带亚热带植物学报,2006,14(4):354-358. |
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| 作者姓名： | 焦旭雯 赵树进 |
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| 作者单位： | 1. 华南理工大学生物科学与工程学院,广州,510640
2. 广州军区广州总医院药学部,广州,510010 |
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| 摘 要： | 流式细胞术(FCM)是根据所测定的各种细胞性质的不同组合,从细胞群体中把某个亚群分选出来,并对它的功能和形态学进行研究或进一步培养分析。流式细胞术具有快速、灵敏和同时进行多参数检测等优点,对其基本原理和在高等植物中的应用进行了介绍。
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| 关 键 词： | 流式细胞术 细胞参数测定 细胞核 原生质体 染色体分拣 综述 |
| 文章编号： | 1005-3395（2006）04-0354-05 |
| 收稿时间： | 2006-01-13 |
| 修稿时间： | 2006-03-18 |
Applications of Flow Cytometry in Higher Plant Research |
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| JIAO Xu-wen,ZHAO Shu-jin.Applications of Flow Cytometry in Higher Plant Research[J].Journal of Tropical and Subtropical Botany,2006,14(4):354-358. |
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| Authors: | JIAO Xu-wen ZHAO Shu-jin |
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| Abstract: | Flow cytometry (FCM) is a current technique for sorting certain cell subset from various cellular associations based on differences in cell's physical and chemical properties, by which the selected cell subset could be further studied in function and morphology. The advantages of this technique include rapidity, sensitivity and synchronization of multi-parameter detection. The principles of FCM are briefly described, and its applications in plant cytoplast, protoplast and chromosome are reviewed. |
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| Keywords: | Flow cytometry Determination of cell parameters Cell nuclei Protoplast Chromosome sorting Review |
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