深山含笑的组织培养和快速繁殖

组织培养深山含笑的实验结果表明：利用休眠芽和种子萌发时实生苗的上胚轴及下胚轴为外植体均能诱导出愈伤组织和不定芽，其中无菌实生苗的上胚轴最易诱导出不定芽，无菌实生苗的下胚轴最易诱导出愈伤组织。外体植体在ＭＳ＋１．０－２．０ｍｇ Ｌ＾－１ ２，４－Ｄ培养基上只产生愈伤组织；在ＭＳ＋３．０ｍｇ Ｌ＾－１ ＢＡ＋０．２ｍｇ Ｌ＾－１ ＮＡＡ培养基上产生较多的不定芽和较多的愈伤组织；在ＭＳ＋２．０ｍｇ Ｌ＾

TISSUE CULTURE AND RAPID PROPAGATION OF MICHELLA MAUDLAE

Explants from dormant bud segments and from epicotyls and hypocotyl of the seedlings of Michelia maudiae Dunn were cultured on MS medium supplemented with various concentrations of NAA, 2,4-D, BA alone or in combination. Medium with 2,4-D could only produce calluses. A large number of adventitious buds and some calluses were observed on medium containing BA (3.0 mg L-1) and NAA (0.2 mg L-1). Calluses from original explants generated a maximum number of shoots in subculture on MS medium supplemented with BA (2.0 mg L-1) and NAA (0.2 mg L-1). Plantlets rooted well on 1/2MS medium containing 0.5 mg L-1 NAA with 1.0% active carbon. The plantlets were transferred to pots containing a perlite/coconut fibre/sand (2: 2: 1) mixture which grew normally with a survival rate of 92%.