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| 甘蔗细胞质型苹果酸脱氢酶基因的克隆及其表达分析 | |
| 引用本文： | 徐景升,阙友雄,颜克伟,唐唯其,许莉萍,高三基,郭晋隆,董萌,陈如凯.甘蔗细胞质型苹果酸脱氢酶基因的克隆及其表达分析[J].热带亚热带植物学报,2012,20(5):455-461. |
| 作者姓名： | 徐景升 阙友雄 颜克伟 唐唯其 许莉萍 高三基 郭晋隆 董萌 陈如凯 |
| 作者单位： | 1. 福建农林大学农业部甘蔗遗传改良重点开放实验室,甘蔗综合研究所,福州350002 2. 福建农林大学生命科学学院,福州,350002 3. 福建农林大学水稻遗传研究所,福州,350002 |
| 基金项目： | 国家自然科学基金项目(30700514); 农业部2010 年引进国际先进农业科学技术“948”计划项目(2010-C21)资助 |
| 摘 要： | 对甘蔗(Saccharum officinarum L.)叶片全长cDNA文库进行测序,获得了1个细胞质型苹果酸脱氢酶(cMDH)基因的全长cDNA序列,命名为Sc-cMDH。生物信息学分析表明,该基因全长1314 bp,开放阅读框为999 bp,编码332个氨基酸。Sc-cMDH与其他植物cMDH的氨基酸序列同源性高达86.5%～97.0%。Sc-cMDH包含典型的NAD+结合基元T11GAAGQI17和催化基元I184WGNH188,还有相当保守的6个半胱氨酸残基,因此推断该基因为细胞质型NAD-MDH。定量PCR分析结果表明,该基因在甘蔗叶片和根中的表达量高于茎。 |
| 关 键 词： | 甘蔗 细胞质型苹果酸脱氢酶基因 实时定量PCR |
| 收稿时间： | 2011/11/14 0:00:00 |
| 修稿时间： | 3/3/2012 12:00:00 AM |
Cloning and Expression Analysis of Cytosolic Malate Dehydrogenase Gene from Sugarcane |
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| XU Jing-sheng,QUE You-xiong,YAN Ke-wei,TANG Wei-qi,XU Li-ping,GAO San-ji,GUO Jin-long,DONG Meng and CHEN Ru-kai.Cloning and Expression Analysis of Cytosolic Malate Dehydrogenase Gene from Sugarcane[J].Journal of Tropical and Subtropical Botany,2012,20(5):455-461. | |
| Authors: | XU Jing-sheng QUE You-xiong YAN Ke-wei TANG Wei-qi XU Li-ping GAO San-ji GUO Jin-long DONG Meng and CHEN Ru-kai |
| Institution: | Key Laboratory of Sugarcane Genetic Improvement, Ministry of Agriculture; Institute of Sugarcane, Fujian Agriculture and Forestry University, China;Key Laboratory of Sugarcane Genetic Improvement, Ministry of Agriculture; Institute of Sugarcane, Fujian Agriculture and Forestry University, China;College of Life Science, Fujian Agriculture and Forestry University, China;Institute of Rice Genetics, Fujian Agriculture and Forestry University, China;Key Laboratory of Sugarcane Genetic Improvement, Ministry of Agriculture; Institute of Sugarcane, Fujian Agriculture and Forestry University, China;Key Laboratory of Sugarcane Genetic Improvement, Ministry of Agriculture; Institute of Sugarcane, Fujian Agriculture and Forestry University, China;Key Laboratory of Sugarcane Genetic Improvement, Ministry of Agriculture; Institute of Sugarcane, Fujian Agriculture and Forestry University, China;Key Laboratory of Sugarcane Genetic Improvement, Ministry of Agriculture; Institute of Sugarcane, Fujian Agriculture and Forestry University, China;Key Laboratory of Sugarcane Genetic Improvement, Ministry of Agriculture; Institute of Sugarcane, Fujian Agriculture and Forestry University, China |
| Abstract: | A full-length cDNA sequence of sugarcane (Saccharum officinarum L.) cytosolic malate dehydrogenase (cMDH) gene, named as Sc-cMDH, was obtained from sugarcane full-length cDNA library by EST sequencing and bioinformatics analysis. The full-length of Sc-cMDH was 1314 bp with 999 bp open reading frame, encoding 332 amino acids. Sc-cMDH shared 86.5%~97.0% of amino acids with cMDH from other 9 species. Sc-cMDH contains typical NAD+ binding motif (T11GAAGQI17) and catalytic motif (I184WGNH188). Real-time PCR analysis showed that the expression of Sc-cMDH was higher in leaves and roots than that in stems of sugarcane. |
| Keywords: | Saccharum officinarum L Cytosolic malate dehydrogenase gene Real-time PCR |
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