| 油莎豆快速繁殖体系的研究 |
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| 引用本文： | 李佳婷,高静阳,孙春雨,万小荣,吴平治,陈雅平,李美茹,姜华武,吴国江.油莎豆快速繁殖体系的研究[J].热带亚热带植物学报,2019,27(4):446-451. |
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| 作者姓名： | 李佳婷 高静阳 孙春雨 万小荣 吴平治 陈雅平 李美茹 姜华武 吴国江 |
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| 作者单位： | 仲恺农业工程学院, 园艺园林学院, 广州 510225;中国科学院华南植物园, 中国科学院植物资源保护与可持续利用重点实验室, 广州 510650,中国科学院华南植物园, 中国科学院植物资源保护与可持续利用重点实验室, 广州 510650;中国科学院大学, 北京 100049,中国科学院华南植物园, 中国科学院植物资源保护与可持续利用重点实验室, 广州 510650;中国科学院大学, 北京 100049,仲恺农业工程学院, 农业与生物学院, 广州 510225,中国科学院华南植物园, 中国科学院植物资源保护与可持续利用重点实验室, 广州 510650,中国科学院华南植物园, 中国科学院植物资源保护与可持续利用重点实验室, 广州 510650,中国科学院华南植物园, 中国科学院植物资源保护与可持续利用重点实验室, 广州 510650,中国科学院华南植物园, 中国科学院植物资源保护与可持续利用重点实验室, 广州 510650,中国科学院华南植物园, 中国科学院植物资源保护与可持续利用重点实验室, 广州 510650 |
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| 基金项目： | 中国科学院重大任务局重点部署项目（ZDRW-ZS-2017-2-3）；中国科学院种子创新研究院项目资助 |
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| 摘 要： | 为建立油莎豆(Cyperus esculentus)的快繁体系,以茎尖为外植体,MS为基本培养基,采用正交设计法研究了6-BA、KT和NAA组合对丛生芽增殖和生根的影响。结果表明,3种生长调节剂对油莎豆丛生芽增殖的影响为6-BA NAA KT,最佳生长调节剂组合为1.0 mg L~(-1) 6-BA+0.2 mg L~(-1) KT,培养4周的增殖系数为7.58。MS培养基为最优生根培养基,生根率可达88.10%,平均生根数为2.04条。这为高效繁育油莎豆优质种苗和种质资源优化奠定基础。
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| 关 键 词： | 油莎豆 无菌苗 快速繁殖 生根 |
| 收稿时间： | 2018/12/3 0:00:00 |
| 修稿时间： | 2019/2/25 0:00:00 |
Studies on Rapid Propagation System of Yellow Nutsedge (Cyperus esculentus L.) in vitro |
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| LI Jia-ting,GAO Jing-yang,SUN Chun-yu,WAN Xiao-rong,WU Ping-zhi,CHEN Ya-ping,LI Mei-ru,JIANG Hua-wu and WU Guo-jiang.Studies on Rapid Propagation System of Yellow Nutsedge (Cyperus esculentus L.) in vitro[J].Journal of Tropical and Subtropical Botany,2019,27(4):446-451. |
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| Authors: | LI Jia-ting GAO Jing-yang SUN Chun-yu WAN Xiao-rong WU Ping-zhi CHEN Ya-ping LI Mei-ru JIANG Hua-wu and WU Guo-jiang |
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| Institution: | College of Horticulture and Landscape Architecture, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China;Key Layboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China,Key Layboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China;University of Chinese Academy of Sciences, Beijing 100049, China,Key Layboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China;University of Chinese Academy of Sciences, Beijing 100049, China,College of Agriculture & Biology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China,Key Layboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China,Key Layboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China,Key Layboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China,Key Layboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China and Key Layboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China |
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| Abstract: | In order to construct rapid propagation system of yellow nutsedge (Cyperus esculentus), the effect of growth regulator combinations, including 6-BA, KT and NAA, was studied by using orthogonal design method with shoot tip as explants on basic medium of MS. The results showed that the effects of growth regulators on proliferation of multiole shoots was in order of 6-BA > NAA > KT. The optimal growth regulator combination was 1.0 mg L-1 6-BA+0.2 mg L-1 KT with proliferation coefficient of 7.58 after cultured for 4 weeks. MS medium was optimal rooting medium with rooting rate of 88.10%, and an average roots was 2.04. So, these would lay a foundation for efficient breeding of high quality seedlings and optimization of germplasm resource of yellow nutsedge. |
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| Keywords: | Cyperus esculentus Aseptic seedling Rapid propagation Root |
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