苏铁蕨配子体发育的研究

用无机培养基培养苏铁蕨(Brainea insignis (Hook.)J.Sm.)的孢子,显微镜下观察记录其孢子萌发及配子体形态发育过程.结果表明:孢子褐色,单裂缝,具周壁,稍褶皱.接种3 d左右孢子萌发,萌发类型为书带蕨型(VittariaType),原叶体发育类型为槲蕨型(Drynaria Type).接种15 d左右发育为片状体.接种25 d左右发育为成熟原叶体,呈心形,其翼面和翼缘均分布有毛状体.精子器由3细胞构成,成熟颈卵器颈部由4列细胞组成,4～5层细胞高.

Studies on the Development of Gametophyte in Brainea insignis

The spores of the Brainea insignis (Hook.) J. Sm. were cultured in the inorganic medium. The spore germination and the gametophytic development were observed by microscope. The results showed that the spores were brown and monolete with a wrinkled perine. The spores germinated about three days after sowing. The spore germination belonged to the Vittaria-Type while the gametophyte development was of the Drynaria-Type. The prothallial plates formed about fifteen days after sowing. The mature cordate prothallus developed twenty-five days after sowing, profuse trichomes bearing superficial and marginal prothallus. Antheridia consisted of three cells. Archegonial neck was usually composed of four rows of cells and four to five cells high at maturity.