铁皮石斛内生真菌次生代谢产物研究

为了解铁皮石斛(Dendrobium officinale)内生真菌Phyllosticta aristolochiicola的次生代谢产物,从该真菌中分离得到15个化合物,经波谱分析分别鉴定为N-methyl-2-pyrolidinone (1)、环-(甘氨酸-L-脯氨酸)(2)、环-(D-丙氨酸-L-脯氨酸)(3)、环-(L-缬氨酸-L-脯氨酸)(4)、环-(L-亮氨酸-L-脯氨酸)(5)、cyclo-(L-Leu-D-4-hydroxyprolinyl)(6)、环-(L-苯丙氨酸-L-脯氨酸)(7)、环-(L-苯丙氨酸-L-4-羟基脯氨酸)(8)、环-(L-酪氨酸-L-脯氨酸)(9)、环-(L-苯丙氨酸-L-亮氨酸)(10)、啤酒甾醇(11)、对羟基苯乙醇(12)、对羟基苯乙酸(13)、(2S,3R)-1-(4-羟基苯基)丁烷-2,3-二醇(14)和(2R,3S)-1-苯基丁烷-2,3-二醇(15)。采用MTS法检测抗肿瘤活性表明,化合物2、10和14对HL-60、A-549、SMMC-7721、MCF-7和SW-480细胞株具有一定的抑制活性。

Study on Secondary Metabolites of Endophytic Fungus Phyllosticta aristolochiicola from Dendrobium officinale

In order to understand the chemical constituents of endophytic fungus Phyllosticta aristolochiicola from Dendrobium officinale, fifteen compounds were isolated. On the basis of spectral data, their structures were identified as N-methyl-2-pyrolidinone (1), cyclo-(glycine-L-proline) (2), cyclo-(D-alanine-L-proline) (3), cyclo-(L-valine-L-proline) (4), cyclo-(L-leucine-L-proline) (5), cyclo-(L-leucine-D-4-hydroxyprolinyl) (6), cyclo-(L-phenylalanine-L-proline) (7), cyclo-(L-4-hydroxyproline-L-phenylalanine) (8), cyclo-(L-tyrosine-L-proline) (9), cyclo-(L-phenylalanine-L-leucine) (10), beer sterol (11), p-hydroxyphenylethanol (12), p-hydroxyphenylacetic acid (13), (2S,3R)-1-(4-hydroxyphenyl)butane-2,3-diol (14), and (2R,3S)-1-phenylbutane-2,3-diol (15). The MTS assay showed that compounds 2, 10 and 14 had weak inhibitory activities against five human tumor cell lines, such as HL-60, A-549, SMMC-7721, MCF-7, and SW-480.