| 植物绿叶粗蛋白在SDS—PAGE中向负极泳动的富含苯丙氨酸的碱性蛋白的测定 |
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| 引用本文： | 梁秀文,黄丽,黄城贵,葛晶,陈红梅,徐杰,董宇亮,郭晋雅,杨净,曾秋莲,徐杰.植物绿叶粗蛋白在SDS—PAGE中向负极泳动的富含苯丙氨酸的碱性蛋白的测定[J].热带亚热带植物学报,2006,14(2):126-129. |
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| 作者姓名： | 梁秀文 黄丽 黄城贵 葛晶 陈红梅 徐杰 董宇亮 郭晋雅 杨净 曾秋莲 徐杰 |
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| 作者单位： | 华南师范大学生命科学学院,广东省植物发育生物工程重点实验室,广州,510631 |
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| 基金项目： | 广东省自然科学基金(021105) |
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| 摘 要： | 提取菜心(BrassicacampestrisL.ssp.chinensis(L.)Makinovar.utillisTsenetLee)和生菜(LactucasativaL.)绿叶粗蛋白,经SDS-变性后作醋酸纤维素薄膜电泳,证实这两种蔬菜含有分别向负极泳动和向正极泳动的蛋白。菜心绿叶粗蛋白经SDS-PAGE电泳后,从负极电泳缓冲液中获得向负极泳动的蛋白,其苯丙氨酸含量为57.05%,碱性/酸性氨基酸残基的比例为3.04,表明是富含苯丙氨酸的碱性蛋白。
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| 关 键 词： | 粗蛋白 醋酸纤维薄膜电泳 负极泳动蛋白 SDS-PAGE 苯丙氨酸 |
| 文章编号： | 1005-3395（2006）02-0126-04 |
| 收稿时间： | 2005-07-14 |
| 修稿时间： | 2005-12-19 |
The Determination of A Phenylalanine-rich Basic Protein from Plant Green Leaves Migrated towards the Cathode in SDS-PAGE |
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| LIANG Xiu-wen,HUANG Li-xi,HUANG Cheng-gui,GE Jing,CHEN Hong-mei,XU Jie,DONG Yu-liang,GUO Jin-y,YANG Jing,ZENG Qiu-lian,XU Jie.The Determination of A Phenylalanine-rich Basic Protein from Plant Green Leaves Migrated towards the Cathode in SDS-PAGE[J].Journal of Tropical and Subtropical Botany,2006,14(2):126-129. |
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| Authors: | LIANG Xiu-wen HUANG Li-xi HUANG Cheng-gui GE Jing CHEN Hong-mei XU Jie DONG Yu-liang GUO Jin-y YANG Jing ZENG Qiu-lian XU Jie |
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| Institution: | College of Life Sciences, South China Normal University,Guangdong Key Lab of Biotechnology for Hant Development, Guangzhou 510631, China |
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| Abstract: | Crude proteins from green leaves of Brassica campestris L.ssp.chinensis(L.) Makino var.utillis Tsen et Lee and Lactuca sativa L.were subjected to acetate cellulose membrane electrophoresis under SDS-denatured conditions.Two groups of protein were observed,one migrated towards the anode,and the other,the cathode.The cathode protein from Brassica campestris ssp.chinensis var.utillis was collected from cathode reservoir after SDS-PAGE,and its amino acid components were determined to have 57.0% phenylalanine,the ratio of basic/ acidic amino acid residues was 3.04,indicating the protein towards cathode was a phenylalanine rich basic protein.It must be emphasized that the protein migrated towards the cathode had never been reported previously. |
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| Keywords: | Crude protein Acetate cellulose membrane electrophoresis Cathode protein SDS-PAGE Phenylalanine |
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