| SSR标记在桉树树种精准鉴定中的应用分析 |
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| 引用本文： | 刘果,谢耀坚,吴志华,陈鸿鹏.SSR标记在桉树树种精准鉴定中的应用分析[J].热带亚热带植物学报,2018,26(2):116-124. |
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| 作者姓名： | 刘果 谢耀坚 吴志华 陈鸿鹏 |
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| 作者单位： | 国家林业局桉树研究开发中心, 广东湛江 524022,国家林业局桉树研究开发中心, 广东湛江 524022,国家林业局桉树研究开发中心, 广东湛江 524022,国家林业局桉树研究开发中心, 广东湛江 524022 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项资金（CAFYBB2016QA018）资助 |
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| 摘 要： | 为精准鉴定桉树种质,利用4个在种内完全保守的SSR位点对28种桉树(Eucalyptus)进行鉴定。根据这4个SSR位点的微卫星重复次数和侧翼序列特异核苷酸组合,构建了28种桉树种间种质资源的鉴定条码,能够精准鉴定9种桉树。这为桉树杂交育种工作提供了生物学依据。
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| 关 键 词： | 桉树 SSR标记 微卫星序列 种质鉴定 |
| 收稿时间： | 2017/6/30 0:00:00 |
| 修稿时间： | 2017/9/13 0:00:00 |
Application of SSR Markers in Precise Identification of Eucalyptus Germplasms |
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| LIU Guo,XIE Yao-jian,WU Zhi-hua and CHEN Hong-peng.Application of SSR Markers in Precise Identification of Eucalyptus Germplasms[J].Journal of Tropical and Subtropical Botany,2018,26(2):116-124. |
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| Authors: | LIU Guo XIE Yao-jian WU Zhi-hua and CHEN Hong-peng |
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| Institution: | Eucalypt Research Centre, State Forest Administration, Zhanjiang 524022, Guangdong, China,Eucalypt Research Centre, State Forest Administration, Zhanjiang 524022, Guangdong, China,Eucalypt Research Centre, State Forest Administration, Zhanjiang 524022, Guangdong, China and Eucalypt Research Centre, State Forest Administration, Zhanjiang 524022, Guangdong, China |
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| Abstract: | In order to precise identification Eucalyptus germplasms, twenty-eight germplasms of Eucalyptus were classified by using 4 SSR loci with complete conservation sequence. According to the multiplicity of microsatellite and the specific bases in associated flanking region (FR) sequences, the identification barcodes among the 28 species of Eucalyptus were constructed, and 9 species of Eucalyptus were precisely identified. Therefore, these would provide the biological basis for hybrid breeding of Eucalyptus. |
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| Keywords: | Eucalyptus SSR marker Microsatellite sequence Germplasm identification |
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