| 利用RAPD标记研究荔枝品种的新缘关系 |
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| 引用本文： | 丁晓东,吕柳新,陈晓静,关雄.利用RAPD标记研究荔枝品种的新缘关系[J].热带亚热带植物学报,2000,8(1):55-59. |
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| 作者姓名： | 丁晓东 吕柳新 陈晓静 关雄 |
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| 作者单位： | [1]东北农业大学园艺系,黑龙江哈尔滨 [2]福建农业大学园艺系,福建福州 |
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| 基金项目： | 国家自然科学基金和福建省自然科学基金资助项目 |
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| 摘 要： | 采用改进的ＣＴＡＢ方法提取３４个荔枝品种的基因组ＤＮＡ为模板,从５０个１０碱基的随机引物中获得了３７个多态性ＲＡＰＤ标记,聚类分析结果表明这３４个荔枝品种可以分成４组,第一组有“状元红”、“元红”、“桂林”、“宋家香”、“台湾荔”、“下番枝”、“东刘一号”、“妃子笑”、“白糖罂”、“香丸”、“绿沙”;第二组有“无栗子”、“水东”、“乌叶”、“蔡家肉丸”、“青壳糯米糍”、“糯米糍”;第三组有“桂味”
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| 关 键 词： | 荔枝 RAPD 聚类分析 品种 亲缘关系 |
| 文章编号： | 1005-3395(2000)01-0049-06 |
| 修稿时间： | 1999年5月20日 |
IDENTIFYING LITCHI CULTIVARS AND EVALUATING THEIR GENETIC RELATIONSHIPS BY RAPD MARKERS |
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| DING Xiao-dong,LU Liu-xin,CHEN Xiao-jing,GUAN Xiong.IDENTIFYING LITCHI CULTIVARS AND EVALUATING THEIR GENETIC RELATIONSHIPS BY RAPD MARKERS[J].Journal of Tropical and Subtropical Botany,2000,8(1):55-59. |
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| Authors: | DING Xiao-dong LU Liu-xin CHEN Xiao-jing GUAN Xiong |
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| Abstract: | In this paper, we used modified CTAB method to extract genomic DNA from litchi for amplifying RAPD markers. We got 37 polymorphic RAPDs out of 259 total RAPDs from 34 litchi cultivars with 50 random primers. The results showed that these 34 cultivars could be divided into 4 groups. Group I included "Zhuangyuanhong", "Yuanhong", "Guilin", "Songjiaxiang", "Taiwanli", "Xiafanzhi", "Dongliu No. l", "Feizixiao", "Baitangying", "Xiangwan", "Lusha". Group II comprised "Wulizi", "Shuidong", "Wuye", "Caijiawanzi", "Qingkenuomici", "Nuomici". Group III included "Guiwei" ,"Guangming","Bailizhi","Chenzi" ,"Lanzhu","Jidi","Tasiniu". Group IV consisted of "Huitoujiang", "Putaoben", "Huaizhi","Nanhaili","Dazao", "Zaohong", "Huoshaoli", "Wuyejiu", "Midingxiang", "Luhebao". The results of cluster suggested that the polymorphism of litchi germplasm was narrower than expected. |
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| Keywords: | Litchi RAPD Cluster analysis |
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