| 三裂叶薯NBS-LRR类抗病基因的筛选鉴定与结构分析 |
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| 引用本文： | 胡韵卓,石媛媛,黄小芳,毕楚韵,周丽香,梁才晓,黄碧芳,许明,林世强,陈选阳.三裂叶薯NBS-LRR类抗病基因的筛选鉴定与结构分析[J].热带亚热带植物学报,2020,28(5):495-504. |
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| 作者姓名： | 胡韵卓 石媛媛 黄小芳 毕楚韵 周丽香 梁才晓 黄碧芳 许明 林世强 陈选阳 |
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| 作者单位： | 福建农林大学, 作物生物技术福建省高校重点实验室, 福州 350002;福建农林大学, 植物保护学院, 福州 350002;福建农林大学, 农学院, 福州 350002;福建农林大学, 作物生物技术福建省高校重点实验室, 福州 350002;福建农林大学, 农学院, 福州 350002;福建农林大学, 生命科学学院, 福州 350002;福建农林大学, 作物生物技术福建省高校重点实验室, 福州 350002;福建农林大学, 生命科学学院, 福州 350002 |
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| 基金项目： | 福建省科技重大专项（2017NZ0002-2,闽农种函[2019]478号）；福建农林大学科技创新平台项目（PTJH12015）资助 |
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| 摘 要： | 为发掘甘薯近缘野生种三裂叶薯(Ipomoea triloba)的NBS-LRR类抗病基因,从基因数据库中对三裂叶薯基因组序列进行了筛选、鉴定和分析。结果表明,从三裂叶薯的98 025个基因中,筛选到282个编码NBS-LRR类蛋白的基因,其中N型80个,NL型83个,CN型28个,CNL型57个,TN型10个,TNL型23个,RN型1个。三裂叶薯的16条染色体上均含有NBS-LRR家族基因,数量最多的染色体含有65个,最少的只有1个。三裂叶薯基因组共有55个基因簇,包含了63.5%的NBS-LRR家族基因。在NBS-LRR抗病基因家族中,CNL和TNL亚家族分别对应到7和11个保守结构域。这为三裂叶薯抗性资源的利用提供了科学参考。
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| 关 键 词： | 三裂叶薯 NBS-LRR R基因 结构域 染色体定位 进化树 |
| 收稿时间： | 2019/12/12 0:00:00 |
| 修稿时间： | 2020/3/9 0:00:00 |
Screening and Identification and Structural Analysis of NBS-LRR Family Genes in Ipomoea triloba |
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| HU Yun-zhuo,SHI Yuan-yuan,HUANG Xiao-fang,BI Chu-yun,ZHOU Li-xiang,LIANG Cai-xiao,HUANG Bi-fang,XU Ming,LIN Shi-qiang,CHEN Xuan-yang.Screening and Identification and Structural Analysis of NBS-LRR Family Genes in Ipomoea triloba[J].Journal of Tropical and Subtropical Botany,2020,28(5):495-504. |
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| Authors: | HU Yun-zhuo SHI Yuan-yuan HUANG Xiao-fang BI Chu-yun ZHOU Li-xiang LIANG Cai-xiao HUANG Bi-fang XU Ming LIN Shi-qiang CHEN Xuan-yang |
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| Institution: | Key Laboratory of Crop Biotechnology, Fujian Agriculture and Forestry University, Fujian Agriculture and Forestry University, Fuzhou 350002, China;College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, China;College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, China;Key Laboratory of Crop Biotechnology, Fujian Agriculture and Forestry University, Fujian Agriculture and Forestry University, Fuzhou 350002, China;College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, China;College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China;Key Laboratory of Crop Biotechnology, Fujian Agriculture and Forestry University, Fujian Agriculture and Forestry University, Fuzhou 350002, China;College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China |
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| Abstract: | In order to exploit the NBS-LRR resistance genes of Ipomoea triloba, a close wild relative of I. batatas, the genome sequence was screened, identified and analyzed by using pertinent software. The results showed that among the 98 025 genes identified from I. triloba, there are 282 genes encoding NBS-LRR proteins, including 80 N type, 83 NL type, 28 CN type, 57 CNL type, 10 TN type, 23 TNL and 1 RN type. NBS-LRR family genes were located in all of 16 chromosomes, each containing 1 to 65 genes. There were 55 gene clusters in genome of I. triloba, containing 63.5% of the NBS-LRR family genes. There were 7 and 11 conservative domains in CNL and TNL subfamilies, respectively. Therefore, these would provide a scientific reference for utilization of resistance resource in I. triloba. |
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| Keywords: | Ipomoea triloba NBS-LRR R gene Domain Chromosomal location Phylogenetic tree |
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