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| 内钙拮抗剂TMB-8对白皮松花粉管细胞壁构建的影响 | |
| 引用本文： | 白秀峰,刘欣欣,吴小琴,张奠湘.内钙拮抗剂TMB-8对白皮松花粉管细胞壁构建的影响[J].热带亚热带植物学报,2011,19(5):463-470. |
| 作者姓名： | 白秀峰 刘欣欣 吴小琴 张奠湘 |
| 作者单位： | 1. 中国科学院华南植物园,中国科学院植物资源保护与可持续利用重点实验室,广州510650;中国科学院研究生院,北京100049 2. 中国科学院华南植物园,中国科学院植物资源保护与可持续利用重点实验室,广州510650;中国科学院植物研究所,中国科学院系统与进化植物学国家重点实验室,北京100093 3. 中国科学院华南植物园,中国科学院植物资源保护与可持续利用重点实验室,广州510650 |
| 基金项目： | 国家自然科学基金项目(31070161); 中国科学院生命科学领域基础前沿研究专项基金(KSCX2-EW-J-28); 中国科学院系统与进化植物学国家重点实验室开放课题基金资助 |
| 摘 要： | 应用荧光显微技术、激光共聚焦扫描显微技术、单克隆抗体免疫荧光标记技术以及傅里叶变换显微红外光谱分析(FTIR)等手段,研究了内钙拮抗剂TMB-8对白皮松花粉管胞内Ca2+分布、花粉管生长以及细胞肇构建等的影响.结果表明,白皮松花粉管经TMB-8处理后,胞内的Ca2+浓度下降,花粉管内典型的Ca2+浓度梯度消失,花粉萌发... |
| 关 键 词： | 白皮松 花粉管 Ca2+ 细胞壁 TMB-8 |
| 收稿时间： | 2010/12/7 0:00:00 |
| 修稿时间： | 2011/3/18 0:00:00 |
Effects of Intracellular Calcium Antagonist, TMB-8, on the Construction of Pollen Tube Wall in Pinus bungeana |
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| BAI Xiu-feng,LIU Xin-xin,WU Xiao-qin and ZHANG Dian-xiang.Effects of Intracellular Calcium Antagonist, TMB-8, on the Construction of Pollen Tube Wall in Pinus bungeana[J].Journal of Tropical and Subtropical Botany,2011,19(5):463-470. | |
| Authors: | BAI Xiu-feng LIU Xin-xin WU Xiao-qin and ZHANG Dian-xiang |
| Institution: | 1. Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, China; 2. Graduate University of Chinese Academy of Sciences, China;1. Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, China; 2. Graduate University of Chinese Academy of Sciences, China;1. Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, China; 2. State Key Laboratory of Systematic and Evolutionary Botany and Evolutionary Botany, Institute of Botany, Ch;Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, China |
| Abstract: | The effects of TMB-8, an intracellular calcium antagonist, on the pollen tube growth and cell wall construction in Pinus bungeana were investigated by means of fluorescence microscopy, laser scanning confocal microscopy, immunofluorescence technique and Fourier transform infra-red (FTIR) microspectroscopy. The results showed that TMB-8 inhibited P. bungeana pollen germination and tube growth in a dose-dependent manner. Normally growing pollen tubes displayed a typical tip-focused cytosolic free Ca2+ concentration gradient, while the Ca2+ concentration gradient dissipated in TMB-8-treated pollen tubes. Cellulose and callose were distributed uniformly in the wall of pollen tubes; acidic pectin was distributed in the tube wall except for the apex while esterified pectin was present only in the apex. In TMB-8-treated tubes, the content of cellulose, acidic pectin and callose increased while esterified pectin decreased in the apex compared with the normal tube wall. It indicated that the intracellular Ca2+ was necessary in the formation of apical cytosolic Ca2+ concentration gradient. Inhibition of intracellular Ca2+ release resulted in the arrest of pollen tube growth by the modification of cell wall composition in P. bungeana pollen tube. |
| Keywords: | Pinus bungeana Pollen tube Ca2+ Cell wall TMB-8 |
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