植物酶的组织和细胞化学定位研究方法

酶是参与植物体内生化反应的特殊蛋白质。在保持活组织和细胞结构完整性的条件下,利用组织化学、细胞化学、免疫学和显微检测等技术研究酶的即位定位,是了解酶在组织、细胞和亚细胞中的分布、活性动态与定量及酶功能等的重要途径。对植物体中酶定位的组织化学和细胞化学方法的概念、原理与研究进展进行了综述,并根据国际酶化学分类编号顺序,分别介绍了25种酶的组织化学染色定位所用的反应介质和染色方法及46种酶的细胞化学定位方法的参考文献。

Methods of In situ Histochemical and Cytochemical Localizations of Plant Enzymes

Enzymes are special proteins that catalyze and regulate a series of life processes in plants. Studies on in situ histochemical, cytochemical, immunological and microscopical localizations of metabolism enzymes in intact plant tissues and cells are important approaches in understanding the distributions, activity dynamics and quantifications, and functions of enzymes within tissues, cells and organelles. The basic concepts, principles and research progresses of histochemical and cytochemical localizations of enzymes were reviewed. According to the international enzymology classification and taxis, the reaction media and staining method of histochemical localizations on 25 plant enzymes and references of cytochemical localizations on 46 plant enzymes were introduced, respectively.