| AgNO3对大白菜子叶芽再生的促进作用 |
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| 引用本文： | 杜虹,庄东红,黄文华.AgNO3对大白菜子叶芽再生的促进作用[J].热带亚热带植物学报,2000,8(2):109-112. |
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| 作者姓名： | 杜虹 庄东红 黄文华 |
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| 作者单位： | 汕头大学理学院生物学系,广东汕头,515063 |
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| 摘 要： | 以大白菜“０２号杂交早皇白”无菌苗的子叶为材料，用附加ＢＡ２．０ｍｇＬ＾－１、ＮＡＡ０．１～１．０ｍｇＬ％－１的ＭＳ培养基培养，能直接放导分化 出芽，最适激素比例为ＢＡ２．０ｍｇＬ＾－１、ＮＡ０．５ｍｇＬ＾－１，芽的分化率为３１．６％，在上述培养基里活加２ｍｇＬ＾－１的ＡｇＮＯ３能使芽的再生频率提高至８６．５％。
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| 关 键 词： | 大白菜 子叶 离体培养 AgNO3 芽再生 |
| 文章编号： | 1005-3395(2000)02-0109-04 |
| 修稿时间： | 1999年8月11日 |
STIMULATION EFFECT OF SILVER NITRATE ON SHOOT REGENERATION IN COTYLEDON TISSUE CULTURE OF BRASSIA CAMPESTRIS |
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| DU Hong,ZHUANG Dong-hong,HUANG Wen-hua.STIMULATION EFFECT OF SILVER NITRATE ON SHOOT REGENERATION IN COTYLEDON TISSUE CULTURE OF BRASSIA CAMPESTRIS[J].Journal of Tropical and Subtropical Botany,2000,8(2):109-112. |
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| Authors: | DU Hong ZHUANG Dong-hong HUANG Wen-hua |
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| Abstract: | A tissue culture system for obtaining high frequency shoot regeneration of Brassica campestris was studied. Shoots could be induced directly from cotyledons cultured on MS medium supplemented with 2.0 mg L-1 BA and 0.5 mg L-1 NAA, the shoot regeneration rate being 31.6%. Addition of 2.0 mg L-1 AgNO3 in the medium could greatly increase the frequency of shoot regeneration, and the shoot regeneration rate reached to 86.5%. |
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| Keywords: | Brassica campestris Cotyledon In vitro culture AgNO3 Shoot regeneration |
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