| 国兰肌动蛋白基因片段的克隆与表达分析 |
| |
| 引用本文： | 田瑞雪,张建霞,张胜鹏,吴坤林,段俊,曾宋君.国兰肌动蛋白基因片段的克隆与表达分析[J].热带亚热带植物学报,2011,19(1):56-62. |
| |
| 作者姓名： | 田瑞雪 张建霞 张胜鹏 吴坤林 段俊 曾宋君 |
| |
| 作者单位： | 1. 中国科学院华南植物园,中国科学院植物资源保护与可持续利用重点实验室,广州510650;中国科学院研究生院,北京100049
2. 中国科学院华南植物园,中国科学院植物资源保护与可持续利用重点实验室,广州510650 |
| |
| 基金项目： | 国家科技支撑计划课题(2008BAC39B05)；广东省农业攻关项目(2009B020201009)；广州市科技支撑项目(2008Z1-E0111) |
| |
| 摘 要： | 根据兰科植物(Orchidaceae)蝴蝶兰(Phdaenopsis)的肌动蛋白基因(Actin)序列设计跨内含子引物,分别以cDNA第一链和基因组DNA为模板,采用RT-PCR和PCR方法从墨兰(Cymbidium sinense)、春兰(C.goeringii)中分离出Actin基因的同源片段.序列分析结果表明:墨...
|
| 关 键 词： | 国兰 肌动蛋白基因 内含子 克隆 表达分析 |
| 收稿时间： | 5/5/2010 12:00:00 AM |
| 修稿时间： | 2010/10/26 0:00:00 |
Cloning and Expression Analysis of Actin Fragment from Cymbidium |
| |
| TIAN Rui-xue,ZHANG Jian-xi,ZHANG Sheng-peng,WU Kun-lin,DUAN Jun and ZENG Song-jun.Cloning and Expression Analysis of Actin Fragment from Cymbidium[J].Journal of Tropical and Subtropical Botany,2011,19(1):56-62. |
| |
| Authors: | TIAN Rui-xue ZHANG Jian-xi ZHANG Sheng-peng WU Kun-lin DUAN Jun and ZENG Song-jun |
| |
| Institution: | 1. Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences; 2. Graduate University of Chinese Academy of Sciences,Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences,1. Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences; 2. Graduate University of Chinese Academy of Sciences,Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences,Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences,South China Botanical Garden,the Chinese Academy of Sciences |
| |
| Abstract: | |
| |
| Keywords: | Cymbidium Actin Introns Cloning Expression analysis |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《热带亚热带植物学报》浏览原始摘要信息 |
| 点击此处可从《热带亚热带植物学报》下载免费的PDF全文 |
|
