光(温)敏核不育水稻花药和小孢子发生的细胞化学

利用细胞化学方法，对光（温）敏核不育水稻农垦585和W6154S的花药和小孢子发生过程的观察结果表明，在可育条件下，其花药组织和小孢子发生过程不论形态结构还是细胞化学变化都基本一致。小孢子母细胞时期的药隔薄壁组织、药壁中层及药室内壁中分布了一些多糖颗粒，但到进入减数分裂时多糖颗粒基本消失。绒毡层在解体前一直富含细胞质，从染色反应看，它表现为小孢子母细胞时期的蛋白质向减数分裂开始后的多糖物质的转变过程。在不育条件下，农垦585在小孢子母细胞时期就出现异常，其败有时间比W6154S要稍早一些。两者最后都表现为典败，但W6154S的花药壁解体较为彻底，只剩下干皱的表皮和药室内壁，而农垦585的花药壁还有多层细胞结构。

CYTOCHEMISTRY OF ANTHER AND MICROSPORE DEVELOPMENT IN PHOTO-/THERMO-SENSITIVE GENIC MALE-STERILE RICE

Cytochemical technique for staining lipids, polysaccharides and proteins in the same section from the tissue embedded in Epon 812 was used to observe the anther and ndcrospore development of photo-/thermo-sensitive genic male-sterile rice Nongken 58S (NK58S) and W6154S. The results are as follows. Under fertile condition, the development of the microspore and anther wall of NK58S was not different from that of W6154S. During the microsporocyte stage, there were some polysaccharide grains in the middle layer and endothecium, but not in tapetal cells. The tapetum was easily staincd with Coomassie Brilliant Blue (CBB). As the microsporocyte went into mciosis, a few polysaccharide grains were present in anther wall. The tapetum was stained actively with Schiff reagent instead of with CBB. Lipid bodies were observed in ma- ture pollen of both NK58S and W6154S. Under abortive condition, although the distribution of polysaccharide grains in anther wall and the staining reaction in tapetum were the same as they were under fertile condition until the separation of uninucleate dricrospore from tetrad, there existed some differences between NK58S and W6154S concerning the whole process of anther and microspore development. Abortion occurred in NK58S earlier than that in W6154S. Even before meiosis, the ndcrosporocyte of NK58S had shown anomalous shape or adhering to the tapetum. At the stage of pollen maturation, the anther wall of W6154S degenerated more thoroughly and consisted of only epidermis and endothecium, however, that of NK58S consisted of epidermis, middle layer and endothecium.