| 葎草雄性连锁的ISSR标记克隆及SCAR标记的建立 |
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| 引用本文： | 高武军,沙涛,姬艳克,邓传良,卢龙斗.葎草雄性连锁的ISSR标记克隆及SCAR标记的建立[J].热带亚热带植物学报,2010,18(3):283-287. |
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| 作者姓名： | 高武军 沙涛 姬艳克 邓传良 卢龙斗 |
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| 作者单位： | 河南师范大学生命科学学院,河南,新乡,453007 |
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| 基金项目： | 国家自然科学基金，河南省自然科学基金 |
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| 摘 要： | 以雌雄异株植物薇草(Humulus scandens L.)为材料,通过优化扩增体系中的退火温度、Mg2+浓度及模板浓度等主要影响因素,利用简单重复序列间扩增标记对其雌雄株性别的基因组差异进行研究.结果表明,62条ISSR引物中有40条引物能扩增出稳定的条带,共产生302条带.引物164扩增出1条雄性连锁标记,经克隆测序,该片段长度为553 bp,AT含量为64.3%,根据测序结果设计特异引物,将该标记转化为稳定性更好的SCAR标记.
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| 关 键 词： | 葎草 雄性连锁 |
| 收稿时间： | 2009/7/13 0:00:00 |
| 修稿时间： | 2009/12/21 0:00:00 |
Clone and Development of ISSR and SCAR Markers Linked to Male Humulus scandens L. |
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| GAO Wu-jun,SHA Tao,JI Yan-ke,DENG Chuan-liang and LU Long-dou.Clone and Development of ISSR and SCAR Markers Linked to Male Humulus scandens L.[J].Journal of Tropical and Subtropical Botany,2010,18(3):283-287. |
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| Authors: | GAO Wu-jun SHA Tao JI Yan-ke DENG Chuan-liang and LU Long-dou |
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| Institution: | College of Life Science,Henan Normal University,College of Life Science,Henan Normal University,College of Life Science,Henan Normal University,College of Life Science,Henan Normal University,College of Life Science,Henan Normal University |
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| Abstract: | The sex differences in Humulus scandens were studied by using inter-simple sequence repeat (ISSR) technique. 40 primes from total 62 primers could produce stable bands, and amplified 302 bands. A male-linked fragment with about 553 bp was amplified by I64 primer which was abundant in AT (64.3%). Then the male-linked marker was converted into SCAR marker. |
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| Keywords: | ISSR SCAR |
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