番茄栽培品种SSR标记和形态标记的遗传多样性分析

比较了SSR标记和形态标记在研究番茄栽培品种遗传多样性上的应用。用7对SSR引物检测了11个番茄栽培品种的遗传多样性,共得到53条带,每一个位点上扩增出2-9条带,平均为6条;品种间遗传相似系数在0.39-0.84之间,平均遗传相似系数为0.60。根据11个形态学性状表型值计算的遗传相似系数在0.27-0.72之间,平均遗传相似系数0.58。用UPGMA进行聚类分析,SSR标记和形态标记都可依果肉颜色和果实大小将供试材料分组,即黄色和红色果肉,樱桃小番茄和大、中果形的混合型;两种方法对番茄栽培品种遗传多样性的评价相近。

Analysis of Genetic Diversity Based on SSR and Morphological Markers among Tomato Cultivars

The analysis of genetic diversity among tomato cultivars was compared by simple sequence repeat(SSR) and morphological markers.Seven SSR primers were used to detect the genetic diversity among 11 tomato cultivars,and total 53 bands were obtained,the average bands per SSR primer pair being 6 with a range from 2 to 9.Mean genetic similarity coefficient among cultivars was 0.60,varying from 0.39 to 0.84.According to 11 phenotype values of morphological characters,the mean genetic similarity coefficient was 0.58,varying from 0.27 to 0.72.Cluster analysis by unweighted pair-group method with arithmetic mean(UPGMA) showed that both SSR and morphological markers could well distinguish the tomato cultivars by the colour of mesocarp and fruit size.The results of the evaluations of genetic diversity in tomato cultivars based on the two methods were similar.