Molecular weight and base composition of DNA isolated from Melanoplus sanguinipes entomopoxvirus |
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| Authors: | WHR Langridge JE Henry |
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| Institution: | 1. Boyce Thompson Institute at Cornell University, Tower Road, Ithaca, New York 14853 USA;2. Entomology Research Division, Agriculture Research Service, U.S. Department of Agriculture, Bozeman, Montana 59715 USA |
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| Abstract: | The DNA genome of the orthopteran entomopoxvirus (EPV) isolated from Melanoplus sanguinipes was released from the virus by treatment with proteinase K and sodium dodecyl sulfate (SDS). The average length of the virus DNA molecule was determined by electron microscopy to be 62.8 μm, corresponding to a molecular weight of 124.3 × 106 daltons (80 kb). The buoyant density of Melanoplus EPV DNA in cesium chloride was calculated to be 1.678 g/cm3, which corresponds to a base ratio of 18.6 mole% guanine + cytosine. |
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| Keywords: | entomopoxvirus DNA electron microscopy buoyant density sedimentation |
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