Phenology, distribution, and host specificity of Solenopsis invicta virus-1 |
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Authors: | Valles Steven M Strong Charles A Oi David H Porter Sanford D Pereira Roberto M Vander Meer Robert K Hashimoto Yoshifumi Hooper-Bùi Linda M Sánchez-Arroyo Hussein Davis Tim Karpakakunjaram Vedham Vail Karen M Fudd Graham L C Briano Juan A Calcaterra Luis A Gilbert Lawrence E Ward Rufina Ward Kenneth Oliver Jason B Taniguchi Glenn Thompson David C |
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Institution: | Center for Medical, Agricultural and Veterinary Entomology, USDA-ARS, 1600 SW 23rd Drive, Gainesville, FL 32608, USA. svalles@gainesville.usda.ufl.edu |
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Abstract: | Studies were conducted to examine the phenology, geographic distribution, and host specificity of the Solenopsis invicta virus-1 (SINV-1). Two genotypes examined, SINV-1 and -1A, exhibited similar seasonal prevalence patterns. Infection rates among colonies of S. invicta in Gainesville, Florida, were lowest from early winter (December) to early spring (April) increasing rapidly in late spring (May) and remaining high through August before declining again in the fall (September/October). Correlation analysis revealed a significant relationship between mean monthly temperature and SINV-1 (p<0.0005, r=0.82) and SINV-1A (p<0.0001, r=0.86) infection rates in S. invicta colonies. SINV-1 was widely distributed among S. invicta populations. The virus was detected in S. invicta from Argentina and from all U.S. states examined, with the exception of New Mexico. SINV-1 and -1A were also detected in other Solenopsis species. SINV-1 was detected in Solenopsis richteri and the S. invicta/richteri hybrid collected from northern Alabama and Solenopsis geminata from Florida. SINV-1A was detected in S. geminata and Solenopsis carolinensis in Florida and the S. invicta/richteri hybrid in Alabama. Of the 1989 arthropods collected from 6 pitfall trap experiments from Gainesville and Williston, Florida, none except S. invicta tested positive for SINV-1 or SINV-1A. SINV-1 did not appear to infect or replicate within Sf9 or Dm-2 cells in vitro. The number of SINV-1 genome copies did not significantly increase over the course of the experiment, nor were any cytopathic effects observed. Phylogenetic analyses of SINV-1/-1A nucleotide sequences indicated significant divergence between viruses collected from Argentina and the U.S. |
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Keywords: | Solenopsis invicta Dicistroviridae RNA virus Host specificity |
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