Age-related changes in the expression of gelatinase and tissue inhibitor of metalloproteinase genes in mandibular condylar,growth plate,and articular cartilage in rats |
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Authors: | Email author" target="_blank">Ichiro?TakahashiEmail author Kazuyuki?Onodera Jin-Wan?Bae Hidetoshi?Mitani Yasuyuki?Sasano Hideo?Mitani |
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Institution: | (1) Division of Orthodontics and Dentofacial Orthopedics, Tohoku University Graduate School of Dentistry, 4-1 Seiryo-machi, Aoba-ku Sendai, 980-8575, Japan;(2) Division of Craniofacial Development and Regeneration, Tohoku University Graduate School of Dentistry, 4-1 Seiryo-machi, Aoba-ku Sendai, 980-8575, Japan |
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Abstract: | Summary Mandibular condylar cartilage acts as both articular and growth plate cartilage during growth, and then becomes articular
cartilage after growth is complete. Cartilaginous extracellular matrix is remodeled continuously via a combination of production,
degradation by matrix metalloproteinases (MMPs), and inhibition of MMP activity by tissue inhibitors of metalloproteinases
(TIMPs). This study attempted to clarify the age-related changes in the mRNA expression patterns of MMP-2, MMP-9, TIMP-1,
TIMP-2, and TIMP-3 in mandibular condylar cartilage in comparison to tibial growth plate and articular cartilage using an
in situ hybridization method in growing and adult rats. MMP-2 and MMP-9 were expressed in a wide range of condylar cartilage cells
during growth, and their expression domains became limited to mature chondrocytes in adults. The patterns of TIMP-1 and TIMP-2
expression were similar to those of MMP-2 and MMP-9 during growth, and were maintained until adulthood. TIMP-3 was localized
to hypertrophic chondrocytes throughout the growth stage. Therefore, we concluded that TIMP-1 and TIMP-2 were general inhibitors
of MMP-2 and MMP-9 in condylar cartilage, while TIMP-3 regulates the collagenolytic degradation of the hypertrophic cartilage
matrix. |
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Keywords: | in situ hybridization matrix degradation MMP TIMP TMJ |
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