脐血造血细胞的体外扩增：2.造血细胞生长因子和培养液更换的作用

研究了造血干细胞生长因子、白介素-3、白介素-6、粒-巨噬细胞集落刺激因子、粒细胞集落刺激因子及红细胞生成素对脐血造血细胞体外培养的影响及其剂量关系，考察了造血细胞因子单独与联合作用对造血细胞体外培养的影响，证实细胞因子组合使用比细胞因子单独使用效果更好，发现SCF+IL-3+IL-6+GM-CSF+G-CSF+EPO组合对总细胞扩增最佳，SCF+IL-3+IL6+GM-CSF组合对CFU-GM扩增最佳。实验发现培养液更换可大大提高脐血造血细胞总数和祖细胞数产出。在每天更换50%培养液下，脐血总细胞数在第三周扩增了27倍，祖细胞数扩增了21倍。

The ex vivo Expansion of Cord Blood Hematopoietic Cells:2. Effects of Cytokines and Medium Exchange Frequency on Expansion

The effects of some cytokines,such as SCF,IL 3,IL 6,GM CSF,G CSF and EPO,on the ex vivo expansion of cord blood hematopoietic cells were investigated.The colony forming depended on the concentrations of those factors and the critical concentrations of G CSF,GM CSF and EPO bringing into full play were,respectively,100,200 and 2 u/mL in semi sold culture on methylcellulose.The effects of the individual and combined cytokines were compared and it was demonstrated that the combined cytokines got the best of the individual.It was found in further study that the combination SCF IL 3 IL 6 GM CSF G CSF EPO was optimum for the expansion of total cells while the combination SCF IL 3 IL 6 GM CSF was the best for the expansion of CFU GM.The effects of four different medium exchange frequencies on the ex vivo expansions of mononuclear cell populations and progenitor cells were experimentally studied.Culture with medium exchange resulted in the expansions of total cells up to 27 folds and that of CFU GM up to 21 folds.