| 门多萨假单胞菌Pseudomonas mendocina NK-01合成褐藻寡糖及其结构鉴定 |
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| 引用本文： | 郭文斌,王淑芳,曹名锋,耿伟涛,宋存江.门多萨假单胞菌Pseudomonas mendocina NK-01合成褐藻寡糖及其结构鉴定[J].生物工程学报,2009,25(9):1366-1370. |
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| 作者姓名： | 郭文斌 王淑芳 曹名锋 耿伟涛 宋存江 |
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| 作者单位： | 1. 南开大学生命科学学院微生物系南开大学生命科学学院分子微生物学与技术教育部重点实验室,天津,300071
2. 南开大学生命科学学院生物活性材料教育部重点实验室,天津,300071 |
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| 基金项目： | 国家高技术研究发展计划(863计划)(No.2007AA06Z323);;天津市基础研究重点项目(No.09JCZDJC18400)资助~~ |
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| 摘 要： | 研究发现门多萨假单胞菌NK-01在菌体内积累中长链聚羟基脂肪酸酯(PHAMCL)的同时也能够合成褐藻寡糖分泌到发酵液中,其产量与培养基的碳氮比有关,高碳氮比有利于褐藻寡糖的合成。本研究利用紫外-可见分光光度法、傅立叶红外光谱分析、1H和13C核磁共振对褐藻寡糖的结构进行了分析鉴定,发现褐藻寡糖的结构是由β-D-甘露糖醛酸、α-L-古洛糖醛酸通过β-(1→4)/α-(1→4)键连接而成的无支化线性多糖,并且在单体的2位或3位羟基上部分乙酰化。凝胶渗透色谱(GPC)对分子量的测定结果为2054。
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| 关 键 词： | 门多萨假单胞菌 发酵 褐藻寡糖 结构鉴定 |
| 收稿时间： | 5/9/2009 12:00:00 AM |
Synthesis and characterization of alginate oligosaccharides produced by Pseudomonas mendocina NK-01 |
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| Wenbin Guo,Shufang Wang,Mingfeng Cao,Weitao Geng and Cunjiang Song.Synthesis and characterization of alginate oligosaccharides produced by Pseudomonas mendocina NK-01[J].Chinese Journal of Biotechnology,2009,25(9):1366-1370. |
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| Authors: | Wenbin Guo Shufang Wang Mingfeng Cao Weitao Geng and Cunjiang Song |
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| Institution: | Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Department of Microbiology, College of Life Sciences, Nankai University, Tianjin 300071, China;The Key Laboratory of Bioactive Materials, Ministry of Education, Nankai University, Tianjin 300071, China;Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Department of Microbiology, College of Life Sciences, Nankai University, Tianjin 300071, China;Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Department of Microbiology, College of Life Sciences, Nankai University, Tianjin 300071, China;Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Department of Microbiology, College of Life Sciences, Nankai University, Tianjin 300071, China |
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| Abstract: | In this study,we found that when Pseudomonas mendocina NK-01 accumulated intracellular carbon reserve,medium chain length poly(3-hydroxyalkanoates),it also synthesized extracellular saccharides,alginate oligosaccharides.The high carbon nitrogen ratio of culture medium facilitated alginate oligosaccharides production.We analyzed the structure of alginate oligosaccharide by Ultraviolet-Visible Spectrophotometry,Fourier Transform Infrared Spectroscopy,1H and 13C of Nuclear Magnetic Resonance,and found that it ... |
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| Keywords: | Pseudomonas mendocina fermentation alginate oligosaccharides structure characterization |
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