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翅鳞伞深层发酵胞外多糖优化研究
引用本文:王允祥 陆兆新<、sup> 吕凤霞.翅鳞伞深层发酵胞外多糖优化研究[J].生物工程学报,2004,20(3):414-422.
作者姓名:王允祥  陆兆新<、sup>  吕凤霞
作者单位:南京农业大学食品科技学院,南京,210095
摘    要:采用PlackettBurman设计(PlackettBurman Design, PB)对影响翅鳞伞[ Pholiota squarrosa (Pers. Ex Fr.) Quel.] AS 5245菌株发酵产糖的内在和外在相关因素进行了筛选,所选取的20个相关因素为葡萄糖、果糖、麦芽糖、酵母膏、胰蛋白胨、KH2PO4、K2HPO4、(NH4)2SO4、NaNO3、FeSO4、MgSO4、MnCl2、ZnCl2、FeCl3、CuSO4·5H2O、维生素B1、起始pH、发酵温度、时间和装液量。在此基础上,再采用响应曲面法(Response Surface Methodology,RSM)对影响发酵产糖的内在关键影响因素酵母膏、果糖、MgSO4、麦芽糖、ZnCl2和发酵基质起始pH值的最佳水平范围作了进一步的研究与探讨,通过对二次多项回归方程求解得知,在上述自变量分别为6.0g/L、11.5g/L、0.5g/L、9.6g/L、38.6mg/L和5.3时,胞外多糖最大预测值为876.32μg/mL发酵醪,此预测可信度不仅被统计分析所验证,也实践所证实。

关 键 词:多糖,  翅鳞伞,  PlackettBurman设计,  响应曲面法(RSM),  中心旋转组合设计
文章编号:1000-3061(2004)03-0414-09
修稿时间:2003年9月25日

Media Optimization for Exopolysaccharide by Pholiota squarrosa (Pers.ex Fr.) Quel.AS 5.245 on Submerged Fermentation
Yun-Xiang Wang,Zhao-Xin Lu,Feng-Xia Lü.Media Optimization for Exopolysaccharide by Pholiota squarrosa (Pers.ex Fr.) Quel.AS 5.245 on Submerged Fermentation[J].Chinese Journal of Biotechnology,2004,20(3):414-422.
Authors:Yun-Xiang Wang  Zhao-Xin Lu  Feng-Xia Lü
Institution:College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Abstract:Our previous work has indicated that mycelium growth and exopolysaccharide accumulation in submerged fermentation by Pholiota squarrosa (Pers. ex Fr.) Quel. AS 5.245 are strongly affected by many internal and external factors, including medium constituents and fermentation conditions. In this study, we use an effective two-phase statistical approach to enhance exopolysaccharide production. In the first phase, Plackett-Burman design was undertaken to evaluate the effects of the twenty factors, i.e., glucose, fructose, maltose, yeast extract, tryptone, K2HPO4, KH2PO4, (NH4)2SO4, NaNO3, FeSO4, MgSO4, MnCl2, ZnCl2, FeCl3, CuSO4.5H2O, vitamin B1, initial pH, the temperature, the medium volume and the duration, to the fermentation. By regression analysis, yeast extract, tryptone, fructose, MgSO4, MnCl2, initial pH and temperature were found to be important for exopolysaccharide production, while glucose, maltose, NaNO3, ZnCl2, vitamin B1, the duration and the volume are important to the mycelium biomass. In the second phase of the optimization process, a response surface methodology (RSM) was used to optimize the above critical internal factors, and to find out the optimal concentration levels and the relationships between these factors. Based on the results of the first phase, a five-level six-factor (yeast extract, fructose, MgSO4, maltose, ZnCl2 and initial pH) central composite rotatable design (CCRD) was employed. By solving the quadratic regression model equation using appropriate statistic methods, the optimal concentrations for obtaining 876.32 microg exopolysaccharide per milliliter of fermentation liquor were calculated as: 6.0g/L yeast extract, 11.5g/L fructose, 0.5g/L MgSO4, 9.6g/L maltose, 38.6mg/L ZnCl2 and with the initial pH 5.3. The experimental data under various conditions have validated the theoretical values.
Keywords:exopolysaccharide  Pholiota squarrosa (Pers  ex Fr  ) Quel    Plackett-Burman design  response surface methodology  central composite rotatable design
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