| 两种合成抗菌肽的结构及抗菌作用机理 |
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| 引用本文： | 杨林,范美华,刘雪珠,武梅,石戈,廖智.两种合成抗菌肽的结构及抗菌作用机理[J].生物工程学报,2011,27(11):1564-1573. |
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| 作者姓名： | 杨林 范美华 刘雪珠 武梅 石戈 廖智 |
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| 作者单位： | 浙江海洋学院海洋科学学院海洋生物资源及分子工程实验室,舟山,316004 |
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| 基金项目： | 浙江省科技厅面上科研农业项目 (Nos. 2008C22026, 2009C32016) 资助。 |
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| 摘 要： | 为深入了解两种新型人工抗菌肽mytilin-derived-peptide-1 (MDP-1) 和mytilin-derived-peptide-2 (MDP-2) 的溶液结构和抗菌机理并探讨两种抗菌肽之间活性差异的结构基础，采用二维核磁共振技术 (2-D NMR) 研究MDP分子的溶液结构；采用透射电镜技术 (Transmitted electron microscopy，TEM) 研究MDP分子对于大肠杆菌和藤黄叠球菌的作用机理。研究结果表明，MDP-1和MDP-2均采取了典型的β-发夹结构，其分子表面具有明显的疏水斑片，其分子中碱性氨基酸突出于分子表面；经MDP分子处理后的大肠杆菌以及藤黄叠球菌均出现细胞壁或细胞膜结构被破坏，并出现膜壁分离以及细胞质内缩现象。我们认为，MDP-1和MDP-2分子中的碱性氨基酸有助于MDP结合细菌表面的带负电荷的基团，同时其分子表面的疏水斑片有助于其插入到细菌细胞膜内；其疏水斑片面积以及碱性氨基酸在分子表面的拓扑结构差异是MDP-1和MDP-2活性差异的主要原因；电镜实验结果表明MDP-1和MDP-2的主要靶标是细菌细胞壁以及细胞膜；上述研究为深入了解MDP分子的结构与功能的关系以及将来基于MDP分子的药物研发奠定了基础。
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| 关 键 词： | mytilin-derived-peptide，人工抗菌肽，核磁共振，溶液结构，电镜分析 |
| 收稿时间： | 2011/4/15 0:00:00 |
Solution structure and antibacterial mechanism of two synthetic antimicrobial peptides |
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| Lin Yang,Meihua Fan,Xuezhu Liu,Mei Wu,Ge Shi and Zhi Liao.Solution structure and antibacterial mechanism of two synthetic antimicrobial peptides[J].Chinese Journal of Biotechnology,2011,27(11):1564-1573. |
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| Authors: | Lin Yang Meihua Fan Xuezhu Liu Mei Wu Ge Shi and Zhi Liao |
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| Institution: | Laboratory of Marine Biology and Molecular Engineering, College of Marine Science, Zhejiang Ocean University, Zhoushan, 316004, China;Laboratory of Marine Biology and Molecular Engineering, College of Marine Science, Zhejiang Ocean University, Zhoushan, 316004, China;Laboratory of Marine Biology and Molecular Engineering, College of Marine Science, Zhejiang Ocean University, Zhoushan, 316004, China;Laboratory of Marine Biology and Molecular Engineering, College of Marine Science, Zhejiang Ocean University, Zhoushan, 316004, China;Laboratory of Marine Biology and Molecular Engineering, College of Marine Science, Zhejiang Ocean University, Zhoushan, 316004, China;Laboratory of Marine Biology and Molecular Engineering, College of Marine Science, Zhejiang Ocean University, Zhoushan, 316004, China |
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| Abstract: | Mytilin-derived-peptide-1(MDP-1) and mytilin-derived-peptide-2(MDP-2) are two truncated decapeptides with reversed sequence synthesized corresponding to the residues 20-29 of mytilin-1(GenBank Accession No.FJ973154) from M.coruscus.The objective of this study is to characterize the structural basis of these two peptides for their antimicrobial activities and functional differences,and to investigate the inhibitory mechanism of MDPs on Escherichia coli and Sarcina lutea.The structures of MDP-1 and MDP-2 in s... |
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| Keywords: | mytilin-derived-peptide artificial antimicrobial peptides nuclear magnetic resonance solution structure transmitted electron microscopy (TEM) |
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