| 日本七鳃鳗Arg-Gly-Asp毒素蛋白Lj-RGD3野生型与RGD全缺失突变体Lj-112的抗血管新生作用 |
| |
| 引用本文： | 王继红,张亚前,吕莉,刘欣,李庆伟.日本七鳃鳗Arg-Gly-Asp毒素蛋白Lj-RGD3野生型与RGD全缺失突变体Lj-112的抗血管新生作用[J].生物工程学报,2011,27(10):1428-1437. |
| |
| 作者姓名： | 王继红 张亚前 吕莉 刘欣 李庆伟 |
| |
| 作者单位： | 1. 辽宁师范大学生命科学学院,大连 116029;辽宁省生物技术与分子药物研发重点实验室,大连 116029
2. 大连医科大学药学院药理教研室,大连,116044 |
| |
| 基金项目： | 国家高技术研究发展计划 (863计划) (No. 2007AA09Z428)，国家自然科学基金 (No. 30770297)，辽宁省教育厅创新团队项目 (No. 2008T102)，大连市重大科技攻关项目 (No. 2010E13SF143)，辽宁省科学技术厅博士启动基金 (No. 20081080)，大连市科学技术局留学回国人员科研基金 (No. 2008J22JH010) 资助。 |
| |
| 摘 要： | 七鳃鳗Arg-Gly-Asp (RGD) 毒素肽Lj-RGD3与富含组氨酸糖蛋白HRG具有序列同源性，而RGD毒素蛋白及HRG都具有抑制血管新生的活性，但作用靶点不同。为研究Lj-RGD3结构与功能的关系，对野生型Lj-RGD3及其RGD全缺失突变体Lj-112进行了抗血管新生功能研究。将3个RGD模体的全缺失突变体基因Lj-112全序列合成后构建于pET-23b载体，对野生型Lj-RGD3及突变体 Lj-112蛋白进行IPTG诱导表达，重组蛋白经组氨酸亲和层析纯化；采用MTT法测定野生型和突变体蛋白对人
|
| 关 键 词： | 日本七鳃鳗，RGD毒素蛋白，富含组氨酸糖蛋白，突变体，血管新生 |
| 收稿时间： | 2011/4/12 0:00:00 |
Anti-angiogenic activities of Lj-RGD3 toxin protein from Lampetra japonica and its mutation protein Lj-112 without RGD motifs |
| |
| Wang Jihong,Zhang Yaqian,Lü Li,Liu Xin,Li Qingwei.Anti-angiogenic activities of Lj-RGD3 toxin protein from Lampetra japonica and its mutation protein Lj-112 without RGD motifs[J].Chinese Journal of Biotechnology,2011,27(10):1428-1437. |
| |
| Authors: | Wang Jihong Zhang Yaqian Lü Li Liu Xin Li Qingwei |
| |
| Institution: | Department of Biological Sciences, Liaoning Normal University, Dalian 116029, China; Liaoning Provincial Key Laboratory of Biotechnology and Drug Discovery, Dalian 116029, China;Department of Biological Sciences, Liaoning Normal University, Dalian 116029, China; Liaoning Provincial Key Laboratory of Biotechnology and Drug Discovery, Dalian 116029, China;Department of Pharmacology, Dalian Medical University, Dalian 116044, China;Department of Biological Sciences, Liaoning Normal University, Dalian 116029, China; Liaoning Provincial Key Laboratory of Biotechnology and Drug Discovery, Dalian 116029, China;Department of Biological Sciences, Liaoning Normal University, Dalian 116029, China; Liaoning Provincial Key Laboratory of Biotechnology and Drug Discovery, Dalian 116029, China |
| |
| Abstract: | Arg-Gly-Asp(RGD)-toxin protein Lj-RGD3 of Lampetra japonica shares homologous with a Histidine-rich glycoprotein(HRG),and both RGD-toxin protein and HRG have antiangiogenic activities with different targets.To study the relationship between the function and the structure of Lj-RGD3,we studied the anti-angiogenic characteristics of both Lj-RGD3 and the mutation named Lj-112 of which three RGD motifs of Lj-RGD3 were deleted.We synthesized the gene of Lj-112,constructed it to the plasmid pET23b,and expressed t... |
| |
| Keywords: | Lampetra japonica RGD-toxin proteins histidine-rich glycoprotein (HRG) mutation angiogenesis |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ |
| 点击此处可从《生物工程学报》浏览原始摘要信息 |
| 点击此处可从《生物工程学报》下载免费的PDF全文 |
|
